Difference between revisions of "20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)"

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===Part 3: Complete cell survival assay===
 
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==Reagents==
 
==Reagents==

Revision as of 20:14, 2 February 2017

20.109(S17): Laboratory Fundamentals of Biological Engineering

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Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

Introduction

nucleic acid purification

reverse transcription

Protocols

Part 1: Workshop with BE Communication Lab

Our communication instructor, Dr. Diana Chien, will join us today for a workshop on transforming a written journal article into a well-defined oral presentation.

Part 2: Prepare samples for quantitative PCR assay

Sp17 20.109 M2D2 qPCR.png

Part 2a: Purify RNA from cells

Part 2b: Generate cDNA

Part 3: Complete cell survival assay

Sp17 20.109 M2D1 cell survival.png

Reagents

Navigation links

Next day: Examine transcript levels in response to DNA damage

Previous day: Complete Western blot and induce DNA damage for survival and quantitative PCR assays
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