Difference between revisions of "20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)"
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==Protocols== | ==Protocols== |
Revision as of 20:32, 1 February 2017
Contents
Introduction
nucleic acid purification
reverse transcription
Protocols
Part 1: Workshop with BE Communication Lab
Our communication instructor, Dr. Diana Chien, will join us today for a workshop on transforming a written journal article into a well-defined oral presentation.
Part 2: Prepare samples for quantitative PCR assay
Part 2a: Purify RNA from cells
Part 2b: Generate cDNA
Part 3: Complete cell survival assay
Reagents
Next day: Examine transcript levels in response to DNA damage
Previous day: Complete Western blot and induce DNA damage for survival and quantitative PCR assays