Difference between revisions of "20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)"
From Course Wiki
Noreen Lyell (Talk | contribs) (→Protocols) |
Noreen Lyell (Talk | contribs) (→Part 1: Workshop with BE Communication Lab) |
||
Line 7: | Line 7: | ||
===Part 1: Workshop with BE Communication Lab=== | ===Part 1: Workshop with BE Communication Lab=== | ||
− | Our communication instructor, Dr. Diana Chien, will join us today for a workshop on | + | Our communication instructor, Dr. Diana Chien, will join us today for a workshop on transforming a written journal article into a well-defined oral presentation. |
===Part 2: Prepare samples for quantitative PCR assay=== | ===Part 2: Prepare samples for quantitative PCR assay=== |
Revision as of 01:07, 1 February 2017
Contents
Introduction
Protocols
Part 1: Workshop with BE Communication Lab
Our communication instructor, Dr. Diana Chien, will join us today for a workshop on transforming a written journal article into a well-defined oral presentation.
Part 2: Prepare samples for quantitative PCR assay
Part 2a: Purify RNA from cells
Part 2b: Generate cDNA
Part 3: Complete cell survival assay
Reagents
Next day: Examine transcript levels in response to DNA damage
Previous day: Complete Western blot and induce DNA damage for survival and quantitative PCR assays