20.109(S10):Post-selection IVT and journal club (Day6)

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20.109(S10): Laboratory Fundamentals of Biological Engineering

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Introduction

You'll begin today by setting up your post-column IVT reactions. Then we will move to room 16-336 for the first round of journal club presentations.

Protocols

Part 1: IVT

Refer to the Day 3 protocol to prepare your IVT Master Mix, except this time shoot for a total volume of 40 μL rather than 80 μL per reaction. (Thus, you will add 6.6 μL of RT-PCR mixture to each reaction instead of 13.1 μL.) Note the time that you put your reactions on the 37 °C heat block.

Part 2: Journal club

We will move to 16-336 for the talks.

For next time

Reagent list

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