Difference between revisions of "20.109(S10):Post-selection IVT and journal club (Day6)"
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(New page: {{Template:20.109(S10)}} ==Introduction== You'll begin today by setting up your post-column IVT reactions. Then we will move to room 16-336 for the first round of journal club presentati...) |
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==Protocols== | ==Protocols== | ||
| − | ===Part 1: === | + | ===Part 1: IVT=== |
| − | ===Part 2: === | + | Refer to the [[20.109%28S10%29:Prepare_RNA_by_IVT_%28Day3%29 |Day 3]] protocol to prepare your IVT Master Mix, except this time shoot for a total volume of 40 μL rather than 80 μL per reaction. (Thus, you will add 6.6 μL of RT-PCR mixture to each reaction instead of 13.1 μL as you did before.) Note the time that you put your reactions on the 37 °C heat block. |
| + | |||
| + | ===Part 2: Journal club=== | ||
| + | |||
| + | We will move to 16-336 for the talks. | ||
==For next time== | ==For next time== | ||
| + | |||
| + | *For everyone... | ||
| + | **No additional lab report homework will be due. However, it is strongly suggested that you continue to work on your report, particularly the RT-PCR figure and experimental schematic. As always, I am happy to provide feedback even when I am not providing a grade. | ||
| + | *If you presented for journal club today... | ||
| + | **You should work on Part III of the computational assignment, and hand in a picture of the full-length 8-12 sequence with the requested highlighting on Day 7. | ||
| + | **An awareness of your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Specifically, describe (in a short phrase or sentence each) two things that you thought you did well, and two that could use improvement. Feel free to include both big-picture and detail-oriented comments. This assignment is due by email within '''48 hours''' after your presentation. | ||
==Reagent list== | ==Reagent list== | ||
| + | |||
| + | Same as Day 3. | ||
Latest revision as of 19:49, 28 July 2015
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Contents
Introduction
You'll begin today by setting up your post-column IVT reactions. Then we will move to room 16-336 for the first round of journal club presentations.
Protocols
Part 1: IVT
Refer to the Day 3 protocol to prepare your IVT Master Mix, except this time shoot for a total volume of 40 μL rather than 80 μL per reaction. (Thus, you will add 6.6 μL of RT-PCR mixture to each reaction instead of 13.1 μL as you did before.) Note the time that you put your reactions on the 37 °C heat block.
Part 2: Journal club
We will move to 16-336 for the talks.
For next time
- For everyone...
- No additional lab report homework will be due. However, it is strongly suggested that you continue to work on your report, particularly the RT-PCR figure and experimental schematic. As always, I am happy to provide feedback even when I am not providing a grade.
- If you presented for journal club today...
- You should work on Part III of the computational assignment, and hand in a picture of the full-length 8-12 sequence with the requested highlighting on Day 7.
- An awareness of your own strengths and weaknesses can often help you improve your future work. After you give your presentation today, write a brief self-evaluation. Specifically, describe (in a short phrase or sentence each) two things that you thought you did well, and two that could use improvement. Feel free to include both big-picture and detail-oriented comments. This assignment is due by email within 48 hours after your presentation.
Reagent list
Same as Day 3.
