Difference between revisions of "20.109(F20): Fall 2020 schedule"

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<font color= #829c58 >'''SCHEDULE DETAILS:'''</font color><br>
 
<font color= #829c58 >'''SCHEDULE DETAILS:'''</font color><br>
−
<font color= #829c58 >'''Lecture times:'''</font color>  T/R 11 - 12 pm <br>
+
<font color= #829c58 >'''Lecture times:'''</font color>  Tuesday (T) and Thursday (R) 11 - 12 pm <br>
−
<font color= #829c58 >'''Laboratory section times:'''</font color>  T/R 1 - 5 pm or W/F 1 - 5 pm <br>
+
<font color= #829c58 >'''Laboratory section times:'''</font color>  Tuesday (T) and Thursday (R) 3 - 5 pm or Wednesday (W) and Friday (F) 3 - 5 pm <br>
  
 
{| border=1px
 
{| border=1px
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|--
 
|--
 
|  
 
|  
−
| T Sept 1   
+
| T/W Sep 1/2  
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>  
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/zsNRMezr119IUp305lzuS54zB6TAaaa81SEaq_dbzoCPSCinH3kBIxY1_DkhoAo?startTime=1598972976000 Video] <br> [[Media:Fa20 Orientation lecture.pdf| Slides]]
−
| [[20.109(F20):Lab tour | Orientation and laboratory tour]]<br>
+
| [[20.109(F20):Lab tour | Orientation and laboratory tour]]<br>[https://mit.zoom.us/j/98495764018 T/R Zoom access], [https://mit.zoom.us/rec/play/u5IuI7uhp2k3HoeQ4QSDAvV9W428Jvms2yAf8_cFn0yzUCICO1TyMOFBZOXpm25FJC_2UtxAo7NLZ-co?continueMode=true T/R prelab video], [[Media:Fa20 M0D0 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/98495764018 W/F Zoom access], [https://mit.zoom.us/rec/play/HbefCTGbxVvI0DBFnq7G6G_o3pL63N9XkaSDDa-sr3VisyHnt88w8igMRUnHSpwF4EKppvSM6I5GVM0.HrMBuJuSWFOXbucE?continueMode=true W/F prelab video], [[Media:Fa20 M0D0 bcm.pdf | W/F prelab slides]] <br>
 
|
 
|
 
|--
 
|--
−
| colspan="7" |
+
| colspan="6" |
 
|--
 
|--
 
| M1D1
 
| M1D1
−
| R Sep 3  
+
| R/F Sep 3/4
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D1 |Learn best practices for mammalian cell culture]]<br>[https://mit.zoom.us/j/98495764018 T/R Zoom access], [https://mit.zoom.us/rec/play/aZy5ACkiu-d_qLAUhwS027wBXBZBX4CK1sIk2AcXyCZaiizRSA4aHOz2UUpVXXDlTZORubSjv38ERVcK.WvDuOFRTdOUiv2l3?continueMode=true T/R prelab video], [[Media:Fa20 M1D1 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/98495764018 W/F Zoom access], [https://mit.zoom.us/rec/play/o7QqrQ2hsRvzyFbjDZ7pbX8HHSdqrtgTkFZtYoArVdze2SbgnbT9jH4oPiYCl4gXeETXfpt2yAdPY1Ir.COOJhZe4nZ6AFNMB?continueMode=true W/F prelab video], [[Media:Fa20 M1D1 nll.pdf| W/F prelab slides]] <br>
−
| <font color= #829c58 >'''Laboratory orientation quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D1|Homework due]]
+
| <font color= #829c58 >'''Orientation quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D1|Homework due]]
 
|--
 
|--
 
| M1D2
 
| M1D2
−
| T Sep 8   
+
| T/W Sep 8/9    
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D2 | Prepare and treat cells for repair foci experiment]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/vXBZQvn71ibjrhhFmwT6aiGIYWkh3NeJkcSdUBF3dD4eECwLbKsTVmyU8fueNuJh.7nv5VzLNbyMue2dK  T/R prelab video], [[Media:Fa20 M1D2 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/nwmcR1eb2vp9Xq6D4Kz52SUggx0uiz7NnD4Sg5HLI_HLdRmSq0zu-2gnUbVxC9G1.vnADhoR0Dgrhe9gM  W/F prelab video], [[Media:Fa20 M1D2 nll.pdf| W/F prelab slides]] <br>
 
| [[20.109(F20):Homework#Due_M1D2|Homework due]]
 
| [[20.109(F20):Homework#Due_M1D2|Homework due]]
 
|--
 
|--
 
| M1D3
 
| M1D3
−
| R Sep 10   
+
| R/F Sep 10/11  
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D3 | Use immunoflourescence staining to assess repair foci experiment]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/eBv9YAQYE6QwbnftLx52nABx4E1TKnc0lBbM7BB_nyQTGgnX-K0o4P5g0-3lbvys.Q9Jkz0-_JZeCGoig  T/R prelab video], [[Media:Fa20 M1D3 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/eJgqA8Ucy8ydiX9AjHRClwAXJ1WrX5buMmMSFwhwg1AGXY8XHGVyUo9mGUxcD9j9.sMcDZpRGid983Xmt  W/F prelab video], [[Media:Fa20 M1D3 nll.pdf| W/F prelab slides]] <br>
 
| [[20.109(F20):Homework#Due_M1D3|Homework due]]
 
| [[20.109(F20):Homework#Due_M1D3|Homework due]]
 
|--
 
|--
 
| M1D4
 
| M1D4
−
| T Sep 15
+
| T/W Sep 15/16
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D4 | Image repair foci experiment and quantify results]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/wa6O0MHbnKKPfnoIs3sV4mjCW0-WpEhhbyq55DXREDeLvHO4s8t-ewjJ_nedj-F6.ko0M24tMPwyQ0kN5  T/R prelab video], [[Media:Fa20 M1D4 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/oT5bdz91C6l7CodSoHxmTxTyiSGgvYOU5qoGxHq7EAnvA-z5iNWgAdqj1zioRurO._8IMEkSefEJ_0U4B  W/F prelab video], [[Media:Fa20 M1D4 nll.pdf| W/F prelab slides]] <br>
−
| [[20.109(F20):Homework#Due_M1D4|Homework due]]
+
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D4|Homework due]]
 
|--
 
|--
 
| M1D5
 
| M1D5
−
| R Sep 17
+
| R/F Sep 17/18
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D5 | Treat cells and perform high-throughput genome damage assay]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/play/eVjNVsv1dKJr2sdLNWfi1KDV5suFqiULCT_y99ZvKqz9PkfY3WSj1BLMU4outG_KHTrxl6fD061ge3nF.3moasScGqvhKKo3r?autoplay=true&continueMode=true&startTime=1600373723000 T/R prelab video], [https://www.dropbox.com/s/ez21c84ddg8ussa/Fa20_M1D5_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/dSDZrHMs5VxJ1fR2qhNKURMtE3hAgi_xFocN6NkXdnROOpPoqUNwHsXsvL_z_8IP.zmP-_UD0LyC_6YYO  W/F prelab video], [[ W/F prelab slides]] <br>
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D5|Homework due]]
+
| [[20.109(F20):Homework#Due_M1D5|Homework due]]
 
|--
 
|--
 
| M1D6
 
| M1D6
−
| T Sep 22  
+
| T/W Sep 22/23
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D6 | Image and analyze high-throughput genome damage assay]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/QLLtl7DggGJFyjr41xQxUqOuaun3LaF2yGPOZgks6kIfMxLEG9FB_2_WItYYq4qy.mKZJit8-qTZmYCgd  T/R prelab video], [https://www.dropbox.com/s/v3chjq5xfjd29ap/Fa20_M1D6_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/YZHssgYuCeNPqI6KkgmXOvyvkizZCdtsPAzzguUK1mp1it9L1uoM2unabFgscwas.sopSMMZZpXui2A5q  W/F prelab video], [[Media:Fa20 M1D6 nll.pdf| W/F prelab slides]] <br>
 
| [[20.109(F20):Homework#Due_M1D6|Homework due]]
 
| [[20.109(F20):Homework#Due_M1D6|Homework due]]
 
|--
 
|--
 
| M1D7
 
| M1D7
−
| R Sep 24
+
| R/F Sep 24/25
−
| [http://be.mit.edu/directory/bevin-p-engelward BE]
+
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|  
+
| [[20.109(F20):M1D7 | Complete data analysis using statistical methods]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/NBQHbV0bOhuRMNcOOEUFwiepv-F4UZZyuuGO7YaXkvdMrrKplUFor1F266qLmASY.etHku2LKdyArjAGI?startTime=1600975551000 T/R prelab video], [[Media:Fa20 M1D7 bcm.pdf | T/R prelab slides]]  <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/Wzarcb-E5YZ763DZHI8Nle4pzWg3hADf5o7FYns34cfY9f2hkcbkLbbfH2Yl7p-s.DxLWwTIzViFalP1K  W/F prelab video], [[Media:Fa20 M1D7 nll.pdf| W/F prelab slides]] <br>
−
| [[20.109(F20):Homework#Due_M1D7|Homework due]]
+
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D7|Homework due]]
 
|--
 
|--
−
| colspan="7" |
+
| colspan="6" |
 
|--
 
|--
 
| M2D1
 
| M2D1
−
| T Sep 29
+
| T/W Sep 29/30
−
| [https://be.mit.edu/directory/jacquin-c-niles JN]
+
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/D7KvRuRfDoWgOBZrq9pL4RhzdZ54s3RPQ6r0O9sJIyvn49akEjNwSZBgOe07AIDv.VXBUfeUJr9qpKdY4?startTime=1601392082000 Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
−
|  
+
| [[20.109(F20):M2D1 | Complete in silico cloning of protein expression plasmid]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/-yY_fdGyNOWmzSPjZt8T-zsZUwdGz_yVM_kjyKWh_5N-iTI8XUSdN2DGZ6gByiXN.OLrxh9xLDDCJi1Dm T/R prelab video], [https://www.dropbox.com/s/695wo1cmg1xyu8c/Fa20_M2D1_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/XZmdbdZu8a1s9OfqqmYCYon8Atmi_cy1JV9yOgm1fTBc_yKy3M3kiVTa4IjhAnCL.ckSRZMh9w-UxS8hV  W/F prelab video], [[ W/F prelab slides]] <br>
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F29):Homework#Due_M2D1|Homework due]]
+
| [[20.109(F20):Homework#Due_M2D1|Homework due]]
 +
|--
 +
| M2D2
 +
| R/F Oct 1/2
 +
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/aU_T1Up_GU0qNWA-O_FqTVEO56f2VgZOCjEiHMn8d-biEwpHOkvzxdS54HjsHhWp.IDL9PosbQMA4Kl02 Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
 +
| [[20.109(F20):M2D2 | Perform protein purification protocol]]<br>[https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/xY3F_44PE9nTrJt9n5CYL8m3-KfH2olQMxQ19504ZU8p9TuD1eGFAmpD_NDIumob.0uF-py1INLNVCnoN W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides]
 +
| [[20.109(F20):Homework#Due_M2D2| Homework due]]<br>[[20.109(F20):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sun, Oct 4 at 10 pm<br>[https://be20109f20blog.blogspot.com/ Blog post due] Mon, Oct 5 at 10 pm
 
|--
 
|--
−
| 2
+
| M2D3
−
| 1
+
| T/W Oct 6/7
−
| T/W Oct 8/9
+
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/4IANFA7azaffZDvl8OvyFof-C8ayGPf12Xp-h9EpgfH80o8gx-Nzf6UWQ9BBh9zY.yfxTtk2D9HJTYMGe Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D1 lecture slides v2.pdf| Lecture slides]]
+
| [[20.109(F20):M2D3 | Assess purity and concentration of purified protein]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/tjXLQ7DEudS4ZXAEDoKGM1U1EoE9liNjUgcoP5yd9pUnlGWRn_x1yBv_Dqz-NgBQ.HgIe0-azNpTnftUB  T/R prelab video], [https://www.dropbox.com/s/5yj54i0n7ul5cx0/Fa20_M2D3_bcm.pdf?dl=0 T/R prelab slides] [[Media:Fa20 protein purification notes.pdf | purification notes]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/6NmHjx_SOVnk2ty_0LL0gpz_ibJgls66XURl2TVxHNHYDInM8oyC2l-NuQ8rY-jg.6LhojnfjplDRvNrJ W/F prelab video], [[Media:Fa20 M2D3 nll.pdf| W/F prelab slides]] <br>
−
| [[20.109(F19):Complete in silico cloning of pdCas9 (Day1)| Complete in silico cloning of pdCas9]]<br>[[Media:M2D1_Fa19_LMmarked.pdf|TR Prelab]]<br> [[Media:M2D1 Fa19 bcm marked.pdf | WF Prelab]]
+
| [[20.109(F20):Homework#Due_M2D3|Homework due]]
−
| [[20.109(F19):Homework#Due_M2D1| Homework due]]<br>
+
 
|--
 
|--
−
| 2
+
| M2D4
−
| 2
+
| R/F Oct 8/9
−
| R/F Oct 10/11
+
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/dGIynwyX4ab21XVtAxIbPgquSYi2TjW9N8JEPkI7gV9dxlnrxZuUXXkKitc_j1gn.289G_71QkFTS1xko  Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D2 lecture slides v2.pdf|Lecture slides]]
+
| [[20.109(F20):M2D4 | Prepare small molecule microarray (SMM) slides with purified protein]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/4k_hF0yO7q4mUMvoML08CuXCF_U-rI8c_rDCZyD4DZcFLeMDVra1Vngpxc7ph5DE.qseaDXdjiXbK8rCn  T/R prelab video], [https://www.dropbox.com/s/hmcfocxagxj9qoj/Fa20_M2D4_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/Wv4slQt_fdsHsBS9BmyUOCW9Kvx187zfrRg1F6A71vZrSos6wloYYql7hI-zDM-5.TRq93V2JGA76NUxk  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides] <br>
−
| [[20.109(F19):Design gRNA sequence for CRISPRi (Day2)| Design gRNA sequence for CRISPRi]]<br>[[Media:M2D2_Fa18_SPmarked.pdf|TR Prelab]]<br>[[Media:Fa19 M2D2 CYK marked.pdf|WF Prelab]]
+
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D4|Homework due]]<br>[[20.109(F20):Mini-presentation| <font color =  #b3251c>'''Mini-presentation due'''</font color>]] Sun, Oct 11 at 10 pm
−
| [[20.109(F19):Homework#Due_M2D2|Homework due]]
+
 
|--
 
|--
 
|  
 
|  
 +
| <font color = 999999>T/W Oct 13/14</font color>
 
|  
 
|  
−
| <font color = 999999>T/W Oct 15/16</font color>
+
| <font color = #0d368e>'''Indigenous Peoples Day holiday'''</font color>
−
|
+
| [[20.109(F20):Data Summary| <font color =  #b3251c>'''Data Summary revision due'''</font color>]] Wed, Oct 14 at 10 pm
−
| <font color = 999999>Columbus day holiday</font color>
+
−
| [[20.109(F19): Data Summary| Data Summary draft due]] Mon, Oct 14 at 10 pm<br>[https://be20109f19.blogspot.com/ Blog post due] Fri, Oct 18 at 10 pm
+
 
|--
 
|--
−
| 2
+
| M2D5
−
| 3
+
| R/F Oct 15/16
−
| R/F Oct 17/18
+
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/FpZHKp2wbeQg_JjcZDHQ8vp2Swy9OviSf75xauagT482Lujhy3iFnYq2U31IF_pH.qFJ44GM9YBVxuRDO Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D3 figures.pptx| Article Figures]]<br>[[Media:Fa19 M2D3 paper discussion slides.pdf| Lecture slides]]
+
| [[20.109(F20):M2D5 | Scan SMM slides to identify putative small molecule binders]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/play/W2Ok0vZcxN4kU_xMz6uCjZUjrXNJWcxGeZYGaYSq5lfRm2iscXp8iGZLR2gHr0xaLqc5A6D66k4C3eOQ.dsuGTbxPFrr38-HT?autoplay=true&continueMode=true&startTime=1602792632000 T/R prelab video], [[Media:Fa20 M2D5 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/qRa5VRy-05qtGJ6TI3nx0yqjht3TUQOMFj72c-FMziY512hUj8ThR9-E14ae3gtB.8dhG663QBfypUeJW  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides] <br>
−
| [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> [[Media:M2D3_Fa18_LM_SP_edit.pdf | TR Prelab]]<br> [[Media:Fa19 M2D3 bcm marked.pdf | WF Prelab]]  
+
| [[20.109(F20):Homework#Due_M2D5|Homework due]]<br>
−
| [[20.109(F19):Homework#Due_M2D3|Homework due]]<br>[[20.109(F19): Mini-presentation| Mini-presentation due]] Sat, Oct 19 at 10 pm
+
 
|--
 
|--
−
| 2
+
|  
−
| 4
+
| T/W Oct 20/21
−
| T/W Oct 22/23
+
|  
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D4 lecture slides.pdf|Lecture slides]]
+
| [[20.109(F20):Journal club presentation| <font color =  #b3251c>'''Journal club presentations'''</font color>]]
−
| [[20.109(F19):Journal club presentation (Day4 and 6)| Journal club I]]
+
 
|  
 
|  
 
|--
 
|--
−
| 2
 
−
| 5
 
−
| R/F Oct 24/25
 
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D5 lecture slides v2.pdf| Lecture slides]]
 
−
| [[20.109(F19):Confirm gRNA sequence in pgRNA (Day5)| Confirm gRNA sequence in pgRNA]]<br> [[Media: M2D5_Fa19_LMmarked.pdf | TR Prelab]]<br> [[Media:Fa19 M2D5 cyk marked.pdf | WF Prelab]]
 
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D5|Homework due]]<br>[[20.109(F19): Data Summary| Data Summary revision due]] Sat, Oct 26 at 10 pm
 
−
|--
 
−
| 2
 
−
| 6
 
−
| T/W Oct 29/30
 
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D6 lecture slides.pdf|Lecture slides]]
 
−
| [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]]
 
 
|  
 
|  
 +
| R/F Oct 22/23
 +
|
 +
| [[20.109(F20):Journal club presentation| <font color =  #b3251c>'''Journal club presentations'''</font color>]]
 +
| [https://be20109f20blog.blogspot.com/ Blog post due] Sun, Oct 25 at 10 pm
 
|--
 
|--
−
| 2
+
| M2D6
−
| 7
+
| T/W Oct 27/28
−
| R/F Oct/Nov 31/1
+
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/vcUUIslbuwKrKE9nCwC4u6CND6Thj0Y3wnats9mDjlhKZ0yUZ5U95xtp2B_5w6ag.heMvV0Dlw-ApbUeo  Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D7 lecture slides.pdf| Lecture slides]]
+
| [[20.109(F20):M2D6 | Analyze SMM data to confirm putative small molecule binders]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/play/8_-yqEhoAEX50nYcC9IB3LX4Rrv0cu6zISlQ57j5HWM687ZHTbUuOs5v3O-HoNT3jthF5RCPbN1GOvaI.BPfydOr9-E1hPjiW?continueMode=true T/R prelab video], [https://www.dropbox.com/s/cfusa6z8z2bvqfs/Fa20_M2D6_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/eempA_2TXzs_mEWr71f_xh8AFjzYp0hN1QXfHY8GoI_FgQpXwhq8kaoPMMaPSPWc.jdX3w_22eE0V57eQ  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides] <br>
−
| [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> [[Media: M2D7_Fa19_LM.pdf | TR Prelab]] <br> [[Media:Fa18 M2D7 bcm marked.pdf | WF Prelab]]
+
| [[20.109(F20):Homework#Due_M2D6|Homework due]]<br>
−
| [[20.109(F19):Homework#Due_M2D7|Homework due]]<br>[https://be20109f19.blogspot.com/ Blog post due] Fri, Nov 1 at 10 pm
+
 
|--
 
|--
−
| 2
+
| M2D7
−
| 8
+
| R/F Oct 29/30
−
| T/W Nov 5/6
+
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/slcV6QJeOUZLOLhuhc0xtGumIQpVvKEIwOwKAc7ijTykj6DL3Ls1tPdzH7GXgQAM.-pR5_VvBe6j_0QKR  Video] <br> [https://www.dropbox.com/sh/dz4jlc1b4kkmiqe/AADQWbknjnrRIbpTXpbY6GoDa?dl=0 Slides]
−
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
+
| [[20.109(F20):M2D7 | Examine putative small molecule binders for common features]] <br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/yX05CxSRGxVxKAE610t1MLgqyS8P4QNWarHrGZF_zRUAtwoz7mewijJBmKd41GDP.O-Dl6JacT-9O5Yua  T/R prelab video], [[Media:Fa20 M2D7 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/4PO_9K9EBOPjHoqvrWb0Kd4lo5_-IumrX9m7EVS6cKO_cizpA2G7rChJlRYnUpUL.f7y2GB8XlhodLOc4  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides] <br>
−
| [[20.109(F19):Measure_fermentation_products_(Day8) | Measure fermentation products]]<br> [[Media: M2D8_Fa19_LMmarked.pdf | TR Prelab]] <br> [[Media:Fa19 M2D8 bcm marked.pdf | WF Prelab]]
+
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D7|Homework due]]<br>
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D8|Homework due]]
+
 
|--
 
|--
−
| colspan="7" |
+
| colspan="6" |
 
|--
 
|--
−
| 3
+
| M3D1
−
| 1
+
| T/W Nov 3/4
−
| R/F Nov 7/8
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/pr1Lh3PdwHqXfXlZX6IZTsdko_o_o2ARRKptp13yX4mhMLcAPG8jloLukAduKGX4.Xalztna5UiIkG7V8  Video] <br> [https://www.dropbox.com/sh/blb2r826you6m90/AABEGALoCTYkFzq6mOwHxv7ua?dl=0 Slides]
−
| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L1 2019.pptx|Lecture slides]]
+
| [[20.109(F20):M3D1 | Research project scope and experimental strategy]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/kA99Bku1xgI1bMCUZxwGi5Hn54XDzor5WDzjgxn8nl9LFk7J3SdugLTWu782U7NI.sHKOjEL7KeBctF6Z T/R prelab video], [https://www.dropbox.com/s/axbpqvvfszxh8ww/Fa20_M3D1_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/uKWJpE6Cbc4Psr5grP8wLbNGp69neHf2u4cj5sACBo64YUgWnchH_VfhYnNkXmF1.fnTViBGY4GxXvII0  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides]<br>
−
| [[20.109(F19):Prepare for cellular thermal shift assay (Day1)| Prepare for cellular thermal shift assay]]<br> [[Media:Fa19 M3D1 prelab slides nllmarked.pdf| TR Prelab]] <br> [[Media:Fa19 M3D1 bcm marked.pdf | WF Prelab]]
+
| [[20.109(F20):Homework#Due_M3D1|Homework due]]<br>
−
| [[20.109(F19):Homework#Due_M3D1|Homework due]] <br>
+
|--
 +
| M3D2
 +
| R/F Nov 5/6
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/l2sVJO3Qc6bNYW-z3IThzEaDAuH5bemK5FgWet6fMMyjR7uIHz_64hYqiUTR9_4W.VDz0bOpLgngz6fef  Video] <br> [https://www.dropbox.com/sh/blb2r826you6m90/AABEGALoCTYkFzq6mOwHxv7ua?dl=0 Slides]
 +
| [[20.109(F20):M3D2 | Align gRNA sequences with genomic targets]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/play/UUyYGXorRbiYzHFfhUWmutGqSG8pRp2ZvlvNrcXsUN7rqautmBwXTnQWzqKEw5XmfwBMwzhr_yDgaJzv.NNEUUQZe3ljsrdEH?continueMode=true T/R prelab video], [[Media:Fa20 M3D2 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/jxvUTG-22lSFcbZmIdjJNcFEzELqyQR2RpgmrUChfvLGsXx0U0cQ17zwYieNORNX.aodbZIjNPmFC6gLa  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides]<br>
 +
| [[20.109(F20):Homework#Due_M3D2|Homework due]]
 
|--
 
|--
−
|
 
 
|
 
|
−
| <font color = 999999>T/W Nov 12/13</font color>
+
| <font color = 999999>T/W Nov 10/11</font color>
 
|  
 
|  
−
| <font color = 999999>Veterans' day holiday</font color>
+
| <font color = #0d368e>'''Veterans day holiday'''</font color>
−
| [[20.109(F19): Research article| Research article due]]  Mon, Nov 11 at 10 pm <br>[https://be20109f19.blogspot.com/ Blog post due] Tue, Nov 12 at 10 pm
+
| [[20.109(F20):Research article| <font color =  #b3251c>'''Research article due'''</font color>]]  Wed, Nov 11 at 10 pm <br>[https://be20109f20blog.blogspot.com/ Blog post due] Thu, Nov 12 at 10 pm
 
|--
 
|--
−
| 3
+
| M3D3
−
| 2
+
| R/F Nov 12/13
−
| R/F Nov 14/15
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/xL1iREP5-pvvL4WMFiafAFfdUlLrZgSZpYsrDaQoqM9d4ymSGQNS4wHFn8nLZ343.rYWIpbR3gIz5RHNz Video] <br> [https://www.dropbox.com/sh/blb2r826you6m90/AABEGALoCTYkFzq6mOwHxv7ua?dl=0 Slides]
−
| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L2 2019.pptx|Lecture slides]]
+
| [[20.109(F20):M3D3 | Analyze ethanol yield data]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/-VSam50XmlC3gGKVNNUKeTMuJsOvtsKegMAMhpJXLXkCVMN66FC9VMjkcgTKqiso.0o60Dm-aWc1aBi94  T/R prelab video], [[Media:Fa20 M3D3 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/PnVMcmzLhvp1Ixn60-GLUpq4tLsOhOMBWhpKNX-FS16ofdPCSaOI_I2GJV96LB5C.93ysE71BmImjmfP5  W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides] <br>
−
| [[20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)| Incubate with ligand and apply heat treatment for protein denaturation]]<br>[[Media:Fa19 M3D2 prelab slides nllmarked.pdf|TR Prelab]] <br> [[Media:191115 prelab CYK marked.pdf | WF Prelab]]
+
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D3|Homework due]]<br>
−
| [[20.109(F19):Homework#Due_M3D2|Homework due]]<br>
+
 
|--  
 
|--  
−
| 3
+
|  
−
| 3
+
| T/W Nov 17/18
−
| T/W Nov 19/20
+
| Research proposal pitches <br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
| [https://be.mit.edu/directory/angela-koehler AK]<br> [[Media:20109 Struntz Lecture 2019.pdf| Lecture slides]]
+
| Develop Research proposal ideas and presentations <br>[https://mit.zoom.us/j/97716476442 T/R Zoom access]<br> [https://mit.zoom.us/j/96606548971 W/F Zoom access]
−
| [[20.109(F19):Begin Western blot analysis (Day3)| Begin Western blot analysis]]<br>[[Media:Fa19 M3D3 prelab slides nllmarked.pdf|TR Prelab]] <br> [[Media:Fa19 M3D3 bcm.pdf | WF Prelab]]
+
|
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D3| Homework due]]
+
 
|--
 
|--
−
| 3
+
| M3D4
−
| 4
+
| R/F Nov 19/20
−
| R/F Nov 21/22
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/hRcnG-d7W0uLxSvu-uCmsSSJE4Fp61UDk2KvT7inIXs7j1EaLLFlEHuWL2zdiokA.lzNri_DxKykem7dN  Video] <br> [https://www.dropbox.com/sh/blb2r826you6m90/AABEGALoCTYkFzq6mOwHxv7ua?dl=0 Slides]
−
| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L4 2019.pdf| Lecture slides]]
+
| [[20.109(F20):M3D4 | Examine features in gRNA-targeted genomic sequences]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/pNSsKobkgbPUhAcAXhpq0vTGUQzXcNlwOEUsljuiH5QYlr9AQTTHYyYduLlpDmzM.eQGA2-xUWlRixevU  T/R prelab video], [[Media:Fa20 M3D4 bcm.pdf | T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/OHr90VrHJpn1swT0rdqUi4eIOM_o3UEwv1NNfM7sUpH-FIxTvzCPO5VnGXhoOaB5.jQ2GB1SAZEzTnbwg W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides]<br>
−
| [[20.109(F19):Complete antibody staining for Western blot analysis (Day4)| Complete antibody staining for Western blot analysis]]<br> [[Media:M3D4 Fa19 bcm marked.pdf | TR Prelab]] <br> [[Media:M3D4WF Fa19 bcm marked.pdf | WF Prelab]]
+
| [[20.109(F20):Homework#Due_M3D4|Homework due]]
−
| [[20.109(F19):Homework#Due_M3D4|Homework due]]
+
 
|--
 
|--
 
|
 
|
−
|
+
| <font color = 999999>T/W Nov 24/25</font color>
−
| T/W Nov 26/27
+
 
|  
 
|  
−
| <font color = #0101DF>'''Lecture: research proposal pitch, but no laboratory'''</font color>
+
| <font color = #0d368e>'''Thanksgiving holiday'''</font color>
 
|  
 
|  
 
|--  
 
|--  
 
|
 
|
 +
| <font color = 999999>R/F Nov 26/27</font color>
 
|
 
|
−
| <font color = 999999>R/F Nov 28/29</font color>
+
| <font color = #0d368e>'''Thanksgiving holiday'''</font color>
−
|
+
−
| <font color = 999999>Thanksgiving holiday</font color>
+
 
|
 
|
 
|--
 
|--
−
| 3
+
| M3D5
−
| 5
+
| T/W Dec 1/2
−
| T/W Dec 3/4 
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> [https://mit.zoom.us/rec/share/MX3xrz40CX1cPyCH9XX2obbbcoggb76P0cT-zd3wgoNTd9lGkpJR_N615Dr_5fVC.1kIDqbQ_C1Nan37i Video] <br> [https://www.dropbox.com/sh/blb2r826you6m90/AABEGALoCTYkFzq6mOwHxv7ua?dl=0 Slides]
−
| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L5 2019.pdf| Lecture slides]]
+
| [[20.109(F20):M3D5 | Design optimized gRNA sequence for enhanced ethanol production]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [https://mit.zoom.us/rec/share/A2J-aHwz_c7rgSNwzgE7tpypaC8EaRqBnRn853YAi6IW8QjL4iPrjhMC8QLw0Q0Y._FBi1l6pxbfsU-_P  T/R prelab video], [https://www.dropbox.com/s/5elod9vbyl7oflx/Fa20_M3D5_bcm.pdf?dl=0 T/R prelab slides] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [https://mit.zoom.us/rec/share/Sds8AE6fF9nlHhIezV7YabS4fIGweNDaDljUbXAYxTd97ePjmSXfgXQfOeYUcRxy.rTu7brbCiSyBID5P W/F prelab video], [https://www.dropbox.com/sh/x76rndhhltlxh8f/AAAddzBoW0xL5h0H6LT1ZipSa?dl=0 W/F prelab slides] <br>
−
| [[20.109(F19):Data analysis and assignment preparation (Day5)| Data analysis and assignment preparation]]<br>[[Media:Fa19 M3D5 prelab slides nllmarked.pdf| TR Prelab]] <br> [[Media:Fa19 M3D5 bcm marked.pdf | WF Prelab]]
+
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D5|Homework due]]
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D5|Homework due]]
+
 
|--
 
|--
 
|  
 
|  
 +
| R/F Dec 3/4
 
|  
 
|  
−
| R/F Dec 5/6
+
| [[20.109(F20):Research proposal presentation| <font color =  #b3251c>'''Research proposal presentations'''</font color>]]
−
|
+
| [https://be20109f20blog.blogspot.com/ Blog post due] Fri, Dec 4 at 10 pm
−
| [[20.109(F19):Research proposal presentation| Research proposal presentations]]
+
−
| [https://be20109f19.blogspot.com/ Blog post due] Fri, Dec 6 at 10 pm
+
 
|--
 
|--
−
| colspan="7" |
+
| colspan="6" |
 
|--
 
|--
 
|  
 
|  
 +
| T Dec 8
 
|  
 
|  
−
| T Dec 10
+
| <font color =  #0d368e>'''Feedback session'''</font color><br> [https://mit.zoom.us/j/93419708072 Zoom access] <br>
−
|
+
| [[20.109(F20):Mini-report | <font color =  #b3251c>'''Mini-report due'''</font color>]] Mon, Dec 7 at 10 pm <br>
−
| <font color =  #0101DF>'''Feedback and celebratory lunch'''</font color>
+
−
| [[20.109(F19): Mini-report | Mini-report due]] Mon, Dec 9 at 10 pm <br>
+
 
|}
 
|}
 
</div>
 
</div>

Latest revision as of 15:57, 3 December 2020

20.109(F20): Laboratory Fundamentals of Biological Engineering

Fa20 banner image v2.png

Fall 2020 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Genomic instability        M2: Drug discovery        M3: Metabolic engineering       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm
Laboratory section times: Tuesday (T) and Thursday (R) 3 - 5 pm or Wednesday (W) and Friday (F) 3 - 5 pm

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Sep 1/2 NLL
Zoom access
Video
Slides 		Orientation and laboratory tour
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
M1D1 R/F Sep 3/4 BE
Zoom access
Learn best practices for mammalian cell culture
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Orientation quiz
Homework due
M1D2 T/W Sep 8/9 BE
Zoom access
Prepare and treat cells for repair foci experiment
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M1D3 R/F Sep 10/11 BE
Zoom access
Use immunoflourescence staining to assess repair foci experiment
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M1D4 T/W Sep 15/16 BE
Zoom access
Image repair foci experiment and quantify results
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Laboratory quiz
Homework due
M1D5 R/F Sep 17/18 BE
Zoom access
Treat cells and perform high-throughput genome damage assay
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M1D6 T/W Sep 22/23 BE
Zoom access
Image and analyze high-throughput genome damage assay
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M1D7 R/F Sep 24/25 BE
Zoom access
Complete data analysis using statistical methods
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Laboratory quiz
Homework due
M2D1 T/W Sep 29/30 JN
Zoom access
Video
Slides 		Complete in silico cloning of protein expression plasmid
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M2D2 R/F Oct 1/2 JN
Zoom access
Video
Slides 		Perform protein purification protocol
W/F Zoom access, W/F prelab video, W/F prelab slides 		Homework due
Data Summary draft due Sun, Oct 4 at 10 pm
Blog post due Mon, Oct 5 at 10 pm
M2D3 T/W Oct 6/7 JN
Zoom access
Video
Slides 		Assess purity and concentration of purified protein
T/R Zoom access, T/R prelab video, T/R prelab slides purification notes
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M2D4 R/F Oct 8/9 JN
Zoom access
Video
Slides 		Prepare small molecule microarray (SMM) slides with purified protein
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Laboratory quiz
Homework due
Mini-presentation due Sun, Oct 11 at 10 pm
T/W Oct 13/14 Indigenous Peoples Day holiday Data Summary revision due Wed, Oct 14 at 10 pm
M2D5 R/F Oct 15/16 JN
Zoom access
Video
Slides 		Scan SMM slides to identify putative small molecule binders
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
T/W Oct 20/21 Journal club presentations
R/F Oct 22/23 Journal club presentations Blog post due Sun, Oct 25 at 10 pm
M2D6 T/W Oct 27/28 JN
Zoom access
Video
Slides 		Analyze SMM data to confirm putative small molecule binders
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Homework due
M2D7 R/F Oct 29/30 JN
Zoom access
Video
Slides 		Examine putative small molecule binders for common features
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Laboratory quiz
Homework due
M3D1 T/W Nov 3/4 NLL
Zoom access
Video
Slides 		Research project scope and experimental strategy
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
 Homework due
M3D2 R/F Nov 5/6 NLL
Zoom access
Video
Slides 		Align gRNA sequences with genomic targets
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
 Homework due
T/W Nov 10/11 Veterans day holiday Research article due Wed, Nov 11 at 10 pm
Blog post due Thu, Nov 12 at 10 pm
M3D3 R/F Nov 12/13 NLL
Zoom access
Video
Slides 		Analyze ethanol yield data
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Laboratory quiz
Homework due
T/W Nov 17/18 Research proposal pitches
Zoom access
Develop Research proposal ideas and presentations
T/R Zoom access
W/F Zoom access
M3D4 R/F Nov 19/20 NLL
Zoom access
Video
Slides 		Examine features in gRNA-targeted genomic sequences
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
 Homework due
T/W Nov 24/25 Thanksgiving holiday
R/F Nov 26/27 Thanksgiving holiday
M3D5 T/W Dec 1/2 NLL
Zoom access
Video
Slides 		Design optimized gRNA sequence for enhanced ethanol production
T/R Zoom access, T/R prelab video, T/R prelab slides
W/F Zoom access, W/F prelab video, W/F prelab slides
Laboratory quiz
Homework due
R/F Dec 3/4 Research proposal presentations Blog post due Fri, Dec 4 at 10 pm
T Dec 8 Feedback session
Zoom access
Mini-report due Mon, Dec 7 at 10 pm
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