Difference between revisions of "20.109(F18):Module 2"
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'''Lecturer:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell]<br> | '''Lecturer:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell]<br> | ||
'''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [http://be.mit.edu/directory/bagnall-josephine Josephine Bagnall]<br> | '''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [http://be.mit.edu/directory/bagnall-josephine Josephine Bagnall]<br> | ||
| − | ''' | + | '''TAs:''' Corban Swain and Jai Padmakumar<br> |
'''Lab manager:''' Hsinhwa Lee <br> | '''Lab manager:''' Hsinhwa Lee <br> | ||
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M2D6: [[20.109(F18):Journal club presentation (Day4 and 6) | Journal club II]]<br> | M2D6: [[20.109(F18):Journal club presentation (Day4 and 6) | Journal club II]]<br> | ||
M2D7: [[20.109(F18):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | M2D7: [[20.109(F18):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | ||
| − | M2D8: [[20.109(F18) | + | M2D8: [[20.109(F18):Measure fermentation products (Day8)| Measure fermentation products]] |
==Assignments== | ==Assignments== | ||
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==References== | ==References== | ||
| − | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression]], | + | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression.]], ''Nature Methods.'' (2013) 8: 2180-2196. |
==Notes for teaching faculty== | ==Notes for teaching faculty== | ||
| − | [[20.109(F18): | + | [[20.109(F18): Prep notes for M2| Prep notes for M2]] |
Latest revision as of 20:45, 4 September 2018
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Josephine Bagnall
TAs: Corban Swain and Jai Padmakumar
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression., Nature Methods. (2013) 8: 2180-2196.
