Difference between revisions of "20.109(F18):Module 2"
Noreen Lyell (Talk | contribs) |
Noreen Lyell (Talk | contribs) (→Notes for teaching faculty) |
||
| Line 38: | Line 38: | ||
==Notes for teaching faculty== | ==Notes for teaching faculty== | ||
| − | [[20.109(F18): TA notes for M2| | + | [[20.109(F18): TA notes for M2| F18 notes for M2]] |
Revision as of 14:44, 28 June 2018
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Josephine Bagnall
TA:
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.
