Difference between revisions of "20.109(F18):Module 2"
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| + | =<center>Module 2</center>= | ||
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| + | '''Lecturer:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell]<br> | ||
| + | '''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [http://be.mit.edu/directory/bagnall-josephine Josephine Bagnall]<br> | ||
| + | '''TA:''' <br> | ||
| + | '''Lab manager:''' Hsinhwa Lee <br> | ||
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| + | ==Overview== | ||
| + | In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in ''E. coli''. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of ''E. coli'' such that either ethanol or acetate production is increased. | ||
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| + | [[Image:Fa16 M2 overview v2.png |thumb|center|700px]] | ||
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| + | <br style="clear:both" /> | ||
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| + | ==Lab links: day by day== | ||
| + | M2D1: [[20.109(F18):Complete in silico cloning (Day1)| Complete ''in silico'' cloning]]<br> | ||
| + | M2D2: [[20.109(F18):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br> | ||
| + | M2D3: [[20.109(F18):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> | ||
| + | M2D4: [[20.109(F18):Journal club presentation (Day4 and 6)| Journal club I]]<br> | ||
| + | M2D5: [[20.109(F18):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br> | ||
| + | M2D6: [[20.109(F18):Journal club presentation (Day4 and 6) | Journal club II]]<br> | ||
| + | M2D7: [[20.109(F18):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> | ||
| + | M2D8: [[20.109(F18)::Measure fermentation products (Day8)| Measure fermentation products]] | ||
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| + | ==Assignments== | ||
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| + | [[20.109(F18): Research article | Research article]]<br> | ||
| + | [[20.109(F18):Journal club presentation (Day4 and 6) | Journal club presentation]] | ||
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| + | ==References== | ||
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| + | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression]], (2013) by Larson ''et al.'' | ||
| + | |||
| + | ==Notes for teaching faculty== | ||
| + | [[20.109(F18): TA notes for M2| F17 notes for M2]] | ||
Revision as of 14:44, 28 June 2018
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Josephine Bagnall
TA:
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.
