Difference between revisions of "20.109(F18):Class data"

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===gRNA_target Sequencing Results===
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====T/R====
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{| border=1px
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|'''Team'''
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|'''pgRNA sequencing'''
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|-
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|red
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|[[Media:Fa18 TR Red M2 seq.zip| files]]
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|-
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|orange
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|[[Media:Fa18 TR Orange M2 seq.zip| files]]
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|-
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|green
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|[[Media:Fa18 TR Green M2 seq.zip| files]]
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|-
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|pink
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|[[Media:Fa18 TR Pink M2 seq.zip| files]]
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|purple
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|[[Media:Fa18 TR Purple M2 seq.zip| files]]
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====W/F====
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{| border=1px
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|'''Team'''
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|'''pgRNA sequencing'''
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|-
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|yellow
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|[[Media:Fa18 WF Red M2 seq.zip| files]]
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|-
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|green
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|[[Media:Fa18 WF Orange M2 seq.zip| files]]
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|-
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|blue
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|[[Media:Fa18 WF Blue M2 seq.zip| files]]
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Revision as of 12:18, 1 November 2018

20.109(F18): Laboratory Fundamentals of Biological Engineering

Fa18 20109 banner image.png

Fall 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Engineering biomaterials              

Module 1

Cell Loading Data

TR and WF cell loading data

CometChip Data

Team Analyzed Data
T/R red Analyzed CometChip data
T/R orange Analyzed CometChip data
T/R green Analyzed CometChip data
T/R pink Analyzed CometChip data
T/R purple Analyzed CometChip data


Team Analyzed Data
W/F yellow Analyzed CometChip data
W/F green Analyzed CometChip data
W/F blue Analyzed CometChip data
W/F pink Analyzed CometChip data


gamma-H2AX Data

Team Recovery time (min) Raw gamma-H2AX Data Analyzed gamma-H2AX Data
T/R red 30 raw data analyzed data
T/R orange 30 raw data analyzed data
T/R green 30 raw data analyzed data
T/R pink 30 raw data analyzed data
T/R purple 60 raw data analyzed data


Team Recovery time (min) Raw gamma-H2AX Data Analyzed gamma-H2AX Data
W/F yellow 30 raw data analyzed data
W/F green 30 raw data analyzed data
W/F blue 60 raw data analyzed data
W/F pink 30 raw data analyzed data

Module 2

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
TR red E pta TGCGCCCGATCAGACTACGACTATC
TR orange E ldhA gttgcaggtacttcttgtcgt
TR green A adhE TACTAAAAAAGTTTAACATTATCA
TR pink A Citrate Synthase (gltA) tgagttttgcttttgtatcagccat
TR purple A ldhA TAGTAGCTTAAATGTGATTCAACAT

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
WF yellow A adhE ATTCGAGCAGATGATTTACTAAAAA
WF green Acetate adhE TTACTAAAAAAGTTTAACATTATCA
WF blue A adhE TTCGAGCAGATGATTTACTAAA

gRNA_target Sequencing Results

T/R

Team pgRNA sequencing
red files
orange files
green files
pink files
purple files

W/F

Team pgRNA sequencing
yellow files
green files
blue files
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