20.109(F18): Laboratory Fundamentals of Biological Engineering
Module 1
Cell Loading Data
TR and WF cell loading data
CometChip Data
gamma-H2AX Data
Module 2
Fermentation product and gene targeted:
T/R
Team
|
Ethanol (E) or Acetate (A)
|
Gene targeted by CRISPRi gRNA
|
gRNA (DNA) sequence (without tag at 3' end)
|
Locus targeted (eg. beginning of gene, putative promoter, -35 region)
|
Target template or nontemplate strand
|
TR red
|
E
|
pta
|
TGCGCCCGATCAGACTACGACTATC
|
|
|
TR orange
|
E
|
ldhA
|
gttgcaggtacttcttgtcgt
|
|
|
TR green
|
A
|
adhE
|
TACTAAAAAAGTTTAACATTATCA
|
|
|
TR pink
|
A
|
Citrate Synthase (gltA)
|
tgagttttgcttttgtatcagccat
|
Beginning of gene
|
Non-template Strand
|
TR purple
|
A
|
ldhA
|
TAGTAGCTTAAATGTGATTCAACAT
|
Locus targeted: -40 upstream region (promoter)
|
Non-template strand
|
|
W/F
Team
|
Ethanol (E) or Acetate (A)
|
Gene targeted by CRISPRi gRNA
|
gRNA (DNA) sequence (without tag at 3' end)
|
Locus targeted (eg. beginning of gene, putative promoter, -35 region)
|
Target template or nontemplate strand
|
WF yellow
|
A
|
adhE
|
ATTCGAGCAGATGATTTACTAAAAA
|
|
|
WF green
|
Acetate
|
adhE
|
TTACTAAAAAAGTTTAACATTATCA
|
|
|
WF blue
|
A
|
adhE
|
TTCGAGCAGATGATTTACTAAA
|
|
|
gRNA_target Sequencing Results
T/R
W/F