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• 20.109(S10):Guidelines for editing OpenWetWare
  ==Sign your work==
  3 KB (494 words) - 19:49, 28 July 2015
• 20.109(S10):Module 2
  ...iscussions during the development of this module, as well as for her prior work in developing a [http://openwetware.org/wiki/20.109:Module_2 related module
  3 KB (497 words) - 19:49, 28 July 2015
• 20.109(S10):Amplify aptamer-encoding DNA (Day1)
  You and your partner may work together on the lab practical. (Note: this will not be the case for future Before starting today's wet lab work, you may want to wipe down your pipettes and your benchtop with 70% ethanol
  10 KB (1,656 words) - 19:49, 28 July 2015
• 20.109(S10):Purify aptamer-encoding DNA (Day2)
  ...ts low viscosity means that high weight percent solutions are tractable to work with, and that the solidified gel remains pliable rather than brittle. HR a ... the work you do in Module 1 in a formal lab report. To help you pace your work, as well as give you feedback early on, you will be required to draft small
  11 KB (1,764 words) - 19:49, 28 July 2015
• 20.109(S10):Prepare RNA by IVT (Day3)
  ... different from DNA in its stability. Consequently it is more difficult to work with RNA in the lab. It is not the techniques themselves that are difficult *Before you begin your experiment clean your work area, removing all clutter. Wipe down the benchtop with warm water then “
  15 KB (2,450 words) - 19:49, 28 July 2015
• 20.109(S10):RNA to DNA by RT-PCR (Day5)
  ...anscription reaction to make more of the RNA aptamer. For this process to work, the 6-5 and 8-12 aptamers must amplify at the same rate, a fact that has b ...o compete with this interaction seems to work. If Prof. Niles’s original work, he used a different RT-PCR kit that did not face this inhibition issue, wh
  7 KB (1,218 words) - 19:49, 28 July 2015
• 20.109(S10):Journal club (Day8)
  ...at 1:30 pm sharp in room 16-336. (Day 6 presentations will begin after lab work is finished, b/w 1:30 and 2 pm.)
  7 KB (1,098 words) - 19:49, 28 July 2015
• 20.109(S10): Assignments
  ...ter in lab, but <font color = CC0000>'''you must submit individual written work (for both daily homeworks and major assignments) and give individual journa ...</font color> We strongly recommend that you plan ahead and space out your work when possible.
  5 KB (682 words) - 19:49, 28 July 2015
• 20.109(S10):Start-up protein engineering (Day1)
  ...s wasting a month of your time repeating experiments already proven not to work or reinventing the wheel. ...As you work, you can ask yourself why these stretches of the protein might work the way that they do, and how they might be changed.
  23 KB (3,840 words) - 19:49, 28 July 2015
• 20.109(S10):Site-directed mutagenesis (Day2)
  ...al of information in order to design mutagenized inverse pericams – nice work! Today you will put your designs into practice. ...reaction, to ensure that all the reagents are working properly. You should work quickly but carefully, and keep your tube in a chilled container at all tim
  13 KB (2,131 words) - 19:49, 28 July 2015
• 20.109(S10):Bacterial amplification of DNA (Day3)
  While the gel runs, you can label the tubes you will need later, work on your notebooks, start the FNT assignment, etc. ...ke your teaching faculty very happy if you contribute to their preparatory work. Please label 2 large glass test tubes with your team color and sample name
  11 KB (1,861 words) - 19:49, 28 July 2015
• 20.109(S10):Prepare expression system (Day4)
  #Incubate on ice for 1 hour. (You might work on parts 2, 4, and 5 of today's protocols now, as well as assemble the mate ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and future app
  22 KB (3,701 words) - 19:49, 28 July 2015
• 20.109(S10):Induce protein and evaluate DNA (Day5)
  As evidenced by Nagai’s work, wild-type inverse pericam is not toxic to BL21(DE3) cells. Although it is ...our cells with IPTG, you will let the resultant protein factories do their work for 2-3 hours. During this time, you will evaluate the DNA from your two X#
  17 KB (2,849 words) - 19:49, 28 July 2015
• 20.109(S10):Assay protein behavior (Day7)
  Only 2-3 groups at a time will work in lab today. Last time you should have signed up to arrive at 1:05, 2:20 o #Now work your way from reservoirs #2 to #12 (highest calcium concentration), and fro
  7 KB (1,187 words) - 19:49, 28 July 2015
• 20.109(S10):Data analysis (Day8)
  ...got last time. Begin by analyzing the wild-type protein as a check on your work (your curve should resemble Nagai's Figure 3L), then move on to your mutant ...t be discouraged if your wild-type values do not exactly match Nagais work, or if there is variation between Parts 1, 2, and 3.
  15 KB (2,419 words) - 19:49, 28 July 2015
• 20.109(S10):Guidelines for oral presentations
  ... elements, including organization, clarity, and proper attribution for the work. *Figure out how to work the lights, slide projector, curtains etc before you begin.
  5 KB (788 words) - 19:49, 28 July 2015
• 20.109(S10):Guidelines for writing up your research
  ...ort by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed. ...motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and future app
  20 KB (3,083 words) - 19:49, 28 July 2015
• 20.109(S10):Start-up biomaterials engineering (Day1)
  ... of the surrounding environment on cell phenotype. In particular, you will work with primary chondrocytes and/or mesenchymal stem cells in 3D gel culture. ...er time. We will also have some mesenchymal stem cells for a few groups to work with, and investigate conditions that promote chondrogenesis. Please see th
  15 KB (2,438 words) - 19:49, 28 July 2015
• 20.109(S10):Initiate cell culture (Day2)
  ...eir knee joints is more similar to that of humans. In this module, we will work with an ''in vitro'' culture model of cartilage-forming cells. ... time however you find useful (FNT assignment, notebook prep, or unrelated work).
  12 KB (1,957 words) - 19:49, 28 July 2015
• 20.109(S10):Testing cell viability (Day3)
  ...r labmates time.''' Reading the protocol in advance will help you work more quickly, and is strongly recommended. ...performing the viability assay, and/or during incubation steps, you should work on Part 3 of today's protocol.
  16 KB (2,677 words) - 22:02, 28 August 2016