20.109(S23):M2D7
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Contents
Introduction
Cell viability assay
The BacTiter-Glo Microbial Cell Viability Assay is a method for quantifying the number of viable cells based on measuring the amount of ATP present. ATP is a proxy for the presence of metabolically active (alive) cells. In this assay, the cells are lysed and ATP is released from the active cells. In a reaction catalyzed by a propriety luciferase enzyme, luciferin, ATP, and oxygen result in oxyluciferin, AMP, PPi, carbon dioxide, and light. The light product is then measured using a luminometer.
Protocols
Part 1: Analyze ICP-OES data
In your laboratory notebook, complete the following:
- What
Part 2: Perform metal tolerance experiment
Reagents list
- WST-8 solution (Abcam)
Next day: Complete data analysis and organize Research article figures