20.109(F17): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11-12 (16-220)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)
MODULE
|
DAY
|
DATE
|
LECTURER
|
LABORATORY EXPERIMENTS
|
ASSIGNMENTS
|
|
|
R/F Sept 7/8
|
NLL Orientation lecture
|
Orientation Prelab T/R Prelab W/F
|
|
|
1
|
1
|
T/W Sept 12/13
|
BE
|
Prepare microwell array and practice tissue culture Prelab T/R Prelab W/F
|
Laboratory orientation quiz Homework due
|
1
|
2
|
R/F Sept 14/15
|
NLL
|
Develop experiment to optimize cell loading Prelab T/R Prelab W/F
|
Homework due
|
1
|
3
|
T/W Sept 19/20
|
BE
|
Evaluate cell loading results Prelab T/R Prelab W/F
|
Homework due
|
1
|
4
|
R/F Sept 21/22
|
BE
|
Test role of biochemical factors in genomic stability Prelab T/R Prelab W/F
|
Homework due
|
1
|
5
|
T/W Sept 26/27
|
BE
|
Complete biochemical experiment and apply chemical treatments for sub-nuclear foci assay Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
|
|
R/F Sept 28/29
|
BE
|
Lecture, but no laboratory Career fair student holiday
|
|
1
|
6
|
T/W Oct 3/4
|
BE
|
Examine sub-nuclear foci abundance to measure DNA damage Prelab T/R Prelab W/F
|
Homework due
|
1
|
7
|
R/F Oct 5/6
|
BE
|
Visualize and analyze data for sub-nuclear foci assay Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
|
|
T/W Oct 10/11
|
|
Columbus day holiday
|
Data Summary draft due Wed, Oct 11 at 10 pm
|
|
2
|
1
|
R/F Oct 12/13
|
NLL Lecture M2D1
|
Complete in silico cloning Prelab T/R Prelab W/F
|
Homework due Mini-presentation due Sat, Oct 14 at 10 pm
|
2
|
2
|
T/W Oct 17/18
|
NLL Lecture M2D2
|
Design gRNA for CRISPRi Prelab T/R Prelab W/F
|
Homework due
|
2
|
3
|
R/F Oct 19/20
|
NLL
|
Generate gRNA plasmid Prelab T/R Prelab W/F
|
Homework due Data Summary revision due Sun, Oct 22 at 10 pm
|
2
|
4
|
T/W Oct 24/25
|
NLL Lecture M2D4
|
Journal club I
|
Blog post due Mon, Oct 23 at 10 pm
|
2
|
5
|
R/F Oct 26/27
|
NLL Lecture M2D5
|
Confirm gRNA sequence Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
2
|
6
|
T/W Oct/Nov 31/1
|
NLL Lecture M2D6
|
Journal club II
|
|
2
|
7
|
R/F Nov 2/3
|
NLL Lecture M2D7
|
Induce CRISPRi system Prelab T/R Prelab W/F
|
Homework due
|
2
|
8
|
T/W Nov 7/8
|
NLL Lecture M2D8
|
Measure fermentation products Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
|
|
R/F Nov 9/10
|
|
Veterans' day holiday
|
|
|
3
|
1
|
T/W Nov 14/15
|
AB
|
Prepare phage materials Prelab T/R Prelab W/F
|
Homework due
|
3
|
2
|
R/F Nov 16/17
|
AB
|
Purify active material for cathode construction Prelab T/R Prelab W/F
|
Homework due
|
|
|
T/W Nov 21/22
|
AB Lecture
|
Lecture, but no laboratory
|
Research article due Mon, Nov 20 at 10 pm Blog post due Tue, Nov 21 at 10 pm
|
|
|
R/F Nov 23/24
|
|
Thanksgiving holiday
|
|
3
|
3
|
T/W Nov 28/29
|
AB
|
Construct cathode with active material Prelab T/R Prelab W/F
|
Laboratory quiz Homework due
|
3
|
4
|
R/F Nov/Dec 30/1
|
AB Lecture
|
Visualize active material using TEM Prelab T/R Prelab W/F
|
Homework due
|
3
|
5
|
T/W Dec 5/6
|
AB
|
Assemble and test batteries
|
Laboratory quiz Homework due
|
3
|
6
|
R/F Dec 7/8
|
|
Research proposal presentations
|
Research proposal presentation slides due Thu/Fri, Dec 7/8 at 1 pm Blog post due Fri, Dec 8 at 10 pm
|
|
|
|
T Dec 12
|
|
Feedback and celebratory lunch
|
Biomaterials engineering mini-report due Mon, Dec 11 at 10 pm Blog post due Tue, Dec 12 at 10pm
|