Difference between revisions of "20.109(S23):Spring 2023 schedule"

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Line 28: Line 28:
 
| M1D1
 
| M1D1
 
| R/F Feb 9/10
 
| R/F Feb 9/10
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D1 | Complete in-silico cloning of protein expression plasmid]]<br>  
 
| [[20.109(S23):M1D1 | Complete in-silico cloning of protein expression plasmid]]<br>  
 
| <font color= #0aa24d >'''Orientation quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D1|Homework due]]
 
| <font color= #0aa24d >'''Orientation quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D1|Homework due]]
Line 34: Line 34:
 
| M1D2
 
| M1D2
 
| T/W Feb 14/15   
 
| T/W Feb 14/15   
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D2 |Perform protein purification protocol]] <br>  
 
| [[20.109(S23):M1D2 |Perform protein purification protocol]] <br>  
 
| [[20.109(S23):Homework#Due_M1D2|Homework due]]
 
| [[20.109(S23):Homework#Due_M1D2|Homework due]]
Line 40: Line 40:
 
| M1D3
 
| M1D3
 
| R/F Feb 16/17   
 
| R/F Feb 16/17   
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D3 |Assess purity and concentration of purified protein]] <br>  
 
| [[20.109(S23):M1D3 |Assess purity and concentration of purified protein]] <br>  
 
| [[20.109(S23):Homework#Due_M1D3|Homework due]]
 
| [[20.109(S23):Homework#Due_M1D3|Homework due]]
Line 52: Line 52:
 
| M1D4
 
| M1D4
 
| R/F Feb 23/24
 
| R/F Feb 23/24
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D4 |Confirm purified protein using Western blot]] <br>  
 
| [[20.109(S23):M1D4 |Confirm purified protein using Western blot]] <br>  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]]
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D4|Homework due]]
Line 58: Line 58:
 
| M1D5
 
| M1D5
 
| T/W Feb/Mar 28/1
 
| T/W Feb/Mar 28/1
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D5 |Image Western blot of purified protein]] <br>   
 
| [[20.109(S23):M1D5 |Image Western blot of purified protein]] <br>   
 
| [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> [[20.109(S23):Research talk| <font color =  #b3251c>'''Research talk due'''</font color>]] XX  
 
| [[20.109(S23):Homework#Due_M1D5|Homework due]] <br> [[20.109(S23):Research talk| <font color =  #b3251c>'''Research talk due'''</font color>]] XX  
Line 64: Line 64:
 
| M1D6
 
| M1D6
 
| R/F Mar 2/3
 
| R/F Mar 2/3
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D6 |Prepare and scan small molecule microarray (SMM) slides]] <br>  
 
| [[20.109(S23):M1D6 |Prepare and scan small molecule microarray (SMM) slides]] <br>  
 
| [[20.109(S23):Homework#Due_M1D6|Homework due]]  
 
| [[20.109(S23):Homework#Due_M1D6|Homework due]]  
Line 70: Line 70:
 
| M1D7
 
| M1D7
 
| T/W Mar 7/8
 
| T/W Mar 7/8
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D7 |Analyze SMM data to identify putative small molecule binders]] <br>  
 
| [[20.109(S23):M1D7 |Analyze SMM data to identify putative small molecule binders]] <br>  
 
| [[20.109(S23):Homework#Due_M1D7|Homework due]]  
 
| [[20.109(S23):Homework#Due_M1D7|Homework due]]  
Line 76: Line 76:
 
| M1D8
 
| M1D8
 
| R/F Mar 9/10
 
| R/F Mar 9/10
| [ AK] <br>  
+
| [http://be.mit.edu/directory/angela-koehler AK] <br>  
 
| [[20.109(S23):M1D8 |Organize Data summary figures and results]] <br>  
 
| [[20.109(S23):M1D8 |Organize Data summary figures and results]] <br>  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D8|Homework due]]  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M1D8|Homework due]]  
Line 82: Line 82:
 
| M2D1
 
| M2D1
 
| T/W Mar 14/15
 
| T/W Mar 14/15
| [ BCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br>  
 
| [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br>  
 
| [[20.109(S23):Homework#Due_M2D1|Homework due]]  
 
| [[20.109(S23):Homework#Due_M2D1|Homework due]]  
Line 88: Line 88:
 
| M2D2
 
| M2D2
 
| R/F Mar 16/17
 
| R/F Mar 16/17
| [ RCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br>  
 
| [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br>  
 
| [[20.109(S23):Homework#Due_M2D2|Homework due]]  
 
| [[20.109(S23):Homework#Due_M2D2|Homework due]]  
Line 94: Line 94:
 
| M2D3
 
| M2D3
 
| T/W Mar 21/22
 
| T/W Mar 21/22
| [ BCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br>  
 
| [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br>  
 
| [[20.109(S23):Homework#Due_M2D3|Homework due]] <br> [[20.109(S23):Data Summary| <font color =  #b3251c>'''Data Summary revision due'''</font color>]] XX <br>  
 
| [[20.109(S23):Homework#Due_M2D3|Homework due]] <br> [[20.109(S23):Data Summary| <font color =  #b3251c>'''Data Summary revision due'''</font color>]] XX <br>  
 
|--
 
|--
 
| M2D4
 
| M2D4
 
|--
 
| M2D5
 
 
| R/F Mar 23/24
 
| R/F Mar 23/24
| [ BCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D4 |Determine transporter mutation and expression]] <br>  
 
| [[20.109(S23):M2D4 |Determine transporter mutation and expression]] <br>  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D4|Homework due]]  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D4|Homework due]]  
Line 118: Line 115:
 
| [[20.109(S23):Journal article presentation| <font color =  #b3251c>'''Journal article presentations'''</font color>]]
 
| [[20.109(S23):Journal article presentation| <font color =  #b3251c>'''Journal article presentations'''</font color>]]
 
|  
 
|  
 +
|--
 +
|
 +
| R/F Apr 6/7
 +
 +
| [[20.109(S23):Journal article presentation| <font color =  #b3251c>'''Journal article presentations'''</font color>]]
 +
| [ Blog post due] Sat, Apr 8 at 10 pm
 
|--
 
|--
 
| M2D5
 
| M2D5
| R/F Apr 6/7
+
| T/W Apr 11/12
| [ BCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D5 |IF analysis and prepare conditions for metal incubation experiment]] <br>  
 
| [[20.109(S23):M2D5 |IF analysis and prepare conditions for metal incubation experiment]] <br>  
 
| [[20.109(S23):Homework#Due_M2D5|Homework due]]  
 
| [[20.109(S23):Homework#Due_M2D5|Homework due]]  
|--
 
| M2D6
 
| T/W Apr 11/12
 
| [ BCM] <br>
 
| [[20.109(S23):Journal article presentation| <font color =  #b3251c>'''Journal article presentations'''</font color>]]
 
| [ Blog post due] Sat, Apr 8 at 10 pm
 
 
|--
 
|--
 
| M2D6
 
| M2D6
 
| R/F Apr 13/14
 
| R/F Apr 13/14
| [ BCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D6 |Analyze ICP-OES data and prepare for cytotoxicity experiment]] <br>  
 
| [[20.109(S23):M2D6 |Analyze ICP-OES data and prepare for cytotoxicity experiment]] <br>  
 
| [[20.109(S23):Homework#Due_M2D5|Homework due]]   
 
| [[20.109(S23):Homework#Due_M2D5|Homework due]]   
Line 145: Line 142:
 
| M2D7
 
| M2D7
 
| R/F Apr 20/21
 
| R/F Apr 20/21
| [ BCM] <br>  
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>  
 
| [[20.109(S23):M2D7 |Determine cell survival of yeast exposed to metal]] <br>  
 
| [[20.109(S23):M2D7 |Determine cell survival of yeast exposed to metal]] <br>  
 
| [[20.109(S23):Homework#Due_M2D7|Homework due]]
 
| [[20.109(S23):Homework#Due_M2D7|Homework due]]
Line 151: Line 148:
 
| M2D8
 
| M2D8
 
| T/W Apr 25/26
 
| T/W Apr 25/26
| [BCM] <br>
+
| [http://be.mit.edu/directory/becky-meyer BCM] <br>
 
| [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br>  
 
| [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br>  
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]
 
| <font color= #0aa24d >'''Laboratory quiz'''</font color> <br> [[20.109(S23):Homework#Due_M2D8|Homework due]

Revision as of 15:42, 26 January 2023

20.109(S23): Laboratory Fundamentals of Biological Engineering

Sp23 banner image v2.png

Spring 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 7/8 NLL
Lecture slides
Orientation and laboratory tour
M1D1 R/F Feb 9/10 AK
Complete in-silico cloning of protein expression plasmid
Orientation quiz
Homework due
M1D2 T/W Feb 14/15 AK
Perform protein purification protocol
Homework due
M1D3 R/F Feb 16/17 AK
Assess purity and concentration of purified protein
Homework due
T/W Feb 21/22 President's Day holiday
M1D4 R/F Feb 23/24 AK
Confirm purified protein using Western blot
Laboratory quiz
Homework due
M1D5 T/W Feb/Mar 28/1 AK
Image Western blot of purified protein
Homework due
Research talk due XX
M1D6 R/F Mar 2/3 AK
Prepare and scan small molecule microarray (SMM) slides
Homework due
M1D7 T/W Mar 7/8 AK
Analyze SMM data to identify putative small molecule binders
Homework due
M1D8 R/F Mar 9/10 AK
Organize Data summary figures and results
Laboratory quiz
Homework due
M2D1 T/W Mar 14/15 BCM
Determine mutagenesis strategy
Homework due
M2D2 R/F Mar 16/17 BCM
Perform site-directed mutagenesis
Homework due
M2D3 T/W Mar 21/22 BCM
Sequence clones and transform into yeast cells
Homework due
Data Summary revision due XX
M2D4 R/F Mar 23/24 BCM
Determine transporter mutation and expression
Laboratory quiz
Homework due
T/W Mar 28/29 - R/F Mar 30/31 Spring Break
T/W Apr 4/5 Journal article presentations
R/F Apr 6/7 Journal article presentations [ Blog post due] Sat, Apr 8 at 10 pm
M2D5 T/W Apr 11/12 BCM
IF analysis and prepare conditions for metal incubation experiment
Homework due
M2D6 R/F Apr 13/14 BCM
Analyze ICP-OES data and prepare for cytotoxicity experiment
Homework due
T/W Apr 18/19 Patriots' Day holiday
M2D7 R/F Apr 20/21 BCM
Determine cell survival of yeast exposed to metal
Homework due
M2D8 T/W Apr 25/26 BCM
Complete data analysis and organize Research article figures
Laboratory quiz
[[20.109(S23):Homework#Due_M2D8|Homework due]
M3D1 R/F Apr 27/28 NLL
Brainstorm ideas for Research proposal presentation
Homework due
Research article due Mon, Nov 21 at 10 pm
Blog post due Tue, Nov 22 at 10 pm
M3D2 T/W May 2/3 AB
Pitch research proposal presentation ideas
M3D3 R/F May 4/5 BE Comm Lab Develop ideas for Research proposal presentation
Homework due
M3D4 T/W May 9/10 NLL
Participate in Research proposal peer reviews
Homework due
R/F May 11/12 Research proposal presentations [ Blog post due] Sat, May 13 at 10 pm
T May 16 Celebration lunch!