Difference between revisions of "20.109(F19): Fall 2019 schedule"
Noreen Lyell (Talk | contribs) |
Noreen Lyell (Talk | contribs) |
||
Line 30: | Line 30: | ||
| T/W Sept 10/11 | | T/W Sept 10/11 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)| | + | | [[20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)| Learn optimal methods for culturing mammalian cells and initiate repair foci analysis experiment]]<br> |
| <font color=#298A08>'''Laboratory orientation quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D1|Homework due]] | | <font color=#298A08>'''Laboratory orientation quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D1|Homework due]] | ||
|-- | |-- | ||
Line 37: | Line 37: | ||
| R/F Sept 12/13 | | R/F Sept 12/13 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F19):Treat cells for gamma-H2AX (Day2)| | + | | [[20.109(F19):Treat cells for gamma-H2AX (Day2)| Perform cell exposures for repair foci experiment and perform high-throughput genome damage pilot]]<br> |
| [[20.109(F19):Homework#Due_M1D2|Homework due]] | | [[20.109(F19):Homework#Due_M1D2|Homework due]] | ||
|-- | |-- | ||
Line 44: | Line 44: | ||
| T/W Sept 17/18 | | T/W Sept 17/18 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)| | + | | [[20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)| Perform immunofluorescence assay for repair foci and optimize high-throughput genome damage assay]]<br> |
| [[20.109(F19):Homework#Due_M1D3|Homework due]] | | [[20.109(F19):Homework#Due_M1D3|Homework due]] | ||
|-- | |-- | ||
Line 58: | Line 58: | ||
| T/W Sept 24/25 | | T/W Sept 24/25 | ||
| Comm Lab | | Comm Lab | ||
− | | [[20.109(F19):Load cells into CometChip and apply treatments (Day4)| | + | | [[20.109(F19):Load cells into CometChip and apply treatments (Day4)| Perform immunofluorescence imaging for repair foci and expose cell for high-throughput genome damage assay]]<br> |
| <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D4|Homework due]] | | <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D4|Homework due]] | ||
|-- | |-- | ||
Line 65: | Line 65: | ||
| R/F Sept 26/27 | | R/F Sept 26/27 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F19):Complete CometChip experiment (Day5)| | + | | [[20.109(F19):Complete CometChip experiment (Day5)| Quantify repair foci via network algorithm and complete high-throughput genome damage assay]]<br> |
| [[20.109(F19):Homework#Due_M1D5|Homework due]] | | [[20.109(F19):Homework#Due_M1D5|Homework due]] | ||
|-- | |-- | ||
Line 72: | Line 72: | ||
| T/W Oct 1/2 | | T/W Oct 1/2 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F19):Image CometChip (Day6)| | + | | [[20.109(F19):Image CometChip (Day6)| Perform quantitative image analysis for the high-throughput genome damage assay]]<br> |
| [[20.109(F19):Homework#Due_M1D6|Homework due]] | | [[20.109(F19):Homework#Due_M1D6|Homework due]] | ||
|-- | |-- | ||
Line 79: | Line 79: | ||
| R/F Oct 3/4 | | R/F Oct 3/4 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE] | | [http://be.mit.edu/directory/bevin-p-engelward BE] | ||
− | | [[20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)| | + | | [[20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)| Complete data analysis and apply statistical methods]]<br> |
| <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D7|Homework due]] | | <font color=#298A08>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D7|Homework due]] | ||
|-- | |-- |
Revision as of 16:23, 9 September 2019
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (16-220)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)