Difference between revisions of "20.109(S23):Class data"
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{{Template:20.109(S23)}} | {{Template:20.109(S23)}} | ||
− | |||
==Module 1: Drug discovery== | ==Module 1: Drug discovery== | ||
+ | ===SDS-PAGE=== | ||
+ | ====T/R==== | ||
+ | Red Team: [[Media:TR Red S23M1D3.jpg]] <br> | ||
+ | Orange Team: [[Media:TR Orange S23M1D3.jpg]] <br> | ||
+ | Yellow Team: [[Media:TR Yellow S23M1D3.jpg]] <br> | ||
+ | Green Team: [[Media:TR Green S23M1D3.jpg]] <br> | ||
+ | Blue Team: [[Media:TR Blue S23M1D3.jpg]] <br> | ||
+ | Pink Team: [[Media:TR Pink S23M1D3.jpg]] <br> | ||
+ | Purple Team: [[Media:TR Purple S23M1D3.jpg]] <br> | ||
+ | |||
+ | ====W/F==== | ||
+ | |||
+ | Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0 | ||
+ | |||
+ | ===BCA=== | ||
+ | ====W/F==== | ||
+ | [[Media: Sp23 W F BCA.xlsx]] | ||
+ | |||
+ | ===Western Blot=== | ||
+ | ====W/F==== | ||
+ | |||
+ | Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf | ||
+ | |||
+ | ===SMM=== | ||
+ | |||
+ | [[Media:Screen results.csv | SMM results]] | ||
==Module 2: Protein engineering== | ==Module 2: Protein engineering== | ||
+ | |||
+ | ===Mutagenesis=== | ||
+ | ====T/R==== | ||
+ | |||
+ | {| border=1px | ||
+ | |'''Team''' | ||
+ | |'''Mutation (eg. S301L)''' | ||
+ | |'''Forward primer''' | ||
+ | |'''Reverse primer''' | ||
+ | |- | ||
+ | |TR red | ||
+ | | Y276C | ||
+ | | TGGCAGATGTgTATTAACACTG | ||
+ | | AGCGTCACTGAACTTTTTC | ||
+ | |- | ||
+ | |TR orange | ||
+ | | Y276C | ||
+ | | TTGGCAGATGtgtATTAACACTG | ||
+ | | GCGTCACTGAACTTTTTC | ||
+ | |- | ||
+ | |TR yellow | ||
+ | | Y276C | ||
+ | | GGCAGATGTAcATTAACACTG | ||
+ | | AAGCGTCACTGAACTTTTTC | ||
+ | |- | ||
+ | |TR green | ||
+ | | Y352C | ||
+ | | GATTGATTGGtgtGCTGATATTATTG | ||
+ | | ATCTTTCTCCCTGTCGAC | ||
+ | |- | ||
+ | |TR blue | ||
+ | | Y276C | ||
+ | | TGGCAGATGTgTATTAACACTG | ||
+ | | AGCGTCACTGAACTTTTTC | ||
+ | |- | ||
+ | |TR pink | ||
+ | | D354C | ||
+ | | TTGGTATGCTtgtATTATTGGTACTGG | ||
+ | | TCAATCATCTTTCTCCCTG | ||
+ | |- | ||
+ | |TR purple | ||
+ | | Y276C | ||
+ | | TGGCAGATGTgTATTAACACTG | ||
+ | | AGCGTCACTGAACTTTTTC | ||
+ | |} | ||
+ | |||
+ | '''T/R Sequencing [[https://www.dropbox.com/sh/tt7s2bmbuwzag23/AADj_lUJ3AirAlREGh9OqL1Za?dl=0 here]]''' | ||
+ | |||
+ | ====W/F==== | ||
+ | |||
+ | |||
+ | {| border=1px | ||
+ | |'''Team''' | ||
+ | |'''Mutation (eg. S301L)''' | ||
+ | |'''Forward primer''' | ||
+ | |'''Reverse primer''' | ||
+ | |||
+ | |- | ||
+ | |WF red | ||
+ | | V281C | ||
+ | | TAACACTGCAtgTGCAGTTTCCCTTC | ||
+ | | ATATACATCTGCCAAGCG | ||
+ | |- | ||
+ | |WF orange | ||
+ | | I349C | ||
+ | | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG | ||
+ | | CCTGTCGACCTTTTTTTAG | ||
+ | |- | ||
+ | |WF yellow | ||
+ | | R405C | ||
+ | | TTTCGTTTTGtgtGAAGTGTACTTCCG | ||
+ | | CCGTCCAAAAACCCAATTAAAC | ||
+ | |- | ||
+ | |WF green | ||
+ | | D271C | ||
+ | | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) | ||
+ | | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) | ||
+ | |- | ||
+ | |WF blue | ||
+ | | Y408C | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |WF pink | ||
+ | | D400C | ||
+ | | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) | ||
+ | | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) | ||
+ | |- | ||
+ | |WF purple | ||
+ | | D350C | ||
+ | | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC | ||
+ | | CTCCCTGTCGACCTTTTTTTAG | ||
+ | |} | ||
+ | |||
+ | '''WF Sequencing [https://www.dropbox.com/sh/jtmnkq24jqdbbsa/AABGRU3EhmCNecalRfzSNkwna?dl=0 here]''' | ||
+ | |||
+ | ===Immunofluorescence Data=== | ||
+ | Pilot Immunofluorescence Data [[https://www.dropbox.com/sh/lul2lkwiv4n5s2c/AADwI02c7chXUc1wiuJrNIvma?dl=0]] | ||
+ | |||
+ | ===ICP-OES data=== | ||
+ | ====T/R==== | ||
+ | Raw ICP-OES data [[https://www.dropbox.com/sh/zofe0u3idj1bpc7/AAC4J0UPo4OYd7oOYLsJdtB8a?dl=0 here]] | ||
+ | |||
+ | {| border=1px | ||
+ | |'''Team''' | ||
+ | |'''Fe (ppm)/ Wavelength''' | ||
+ | |'''Cd (ppm)/ Wavelength''' | ||
+ | |'''O.D.''' | ||
+ | |- | ||
+ | |TR red | ||
+ | | 2.62 ppm / 240.489 nm | ||
+ | | 7.58 ppm / 361.287 nm | ||
+ | | 1.889 | ||
+ | |- | ||
+ | |TR orange | ||
+ | | 4.29 ppm / 261.382 nm | ||
+ | | 11.16 ppm / 326.105 nm | ||
+ | | 1.566 | ||
+ | |- | ||
+ | |TR yellow | ||
+ | | 3.70 ppm / 261.382 nm | ||
+ | | 11.35 ppm / 346.766 nm | ||
+ | | 1.529 | ||
+ | |- | ||
+ | |TR green | ||
+ | | 4.58 ppm / 238.863 nm | ||
+ | | 11.50 ppm / 479.991 nm | ||
+ | | 1.495 | ||
+ | |- | ||
+ | |TR blue | ||
+ | | 4.66 ppm / 259.837 nm | ||
+ | | 11.52 ppm /479.999 nm | ||
+ | | 1.546 | ||
+ | |- | ||
+ | |TR pink | ||
+ | | 4.03ppm / 238.863nm | ||
+ | | 10.52ppm / 232.929nm | ||
+ | | 1.581 | ||
+ | |- | ||
+ | |TR purple | ||
+ | | 4.19 ppm / 238.204 nm | ||
+ | | 10.90 ppm / 223.986 nm | ||
+ | | 1.458 | ||
+ | |- | ||
+ | |TR WT | ||
+ | | | ||
+ | | | ||
+ | | 1 | ||
+ | |- | ||
+ | |TR Fet4 | ||
+ | | | ||
+ | | | ||
+ | | 1.28 | ||
+ | |} | ||
+ | |||
+ | ====W/F==== | ||
+ | Raw ICP-OES data [[https://www.dropbox.com/sh/3c74r7t8xqp81i6/AAD_7yAiRf_XWSwpH_jGi03Oa?dl=0 here]] | ||
+ | |||
+ | {| border=1px | ||
+ | |'''Team''' | ||
+ | |'''Fe (ppm)/ Wavelength''' | ||
+ | |'''Cd (ppm)/ Wavelength''' | ||
+ | |'''O.D.''' | ||
+ | |||
+ | |- | ||
+ | |WF red | ||
+ | | 4.09 / 238.863 | ||
+ | | 10.99 /213.439 | ||
+ | | 1.486 | ||
+ | |- | ||
+ | |WF orange | ||
+ | | 3.73 / 238.204 | ||
+ | | 11.63 / 226.502 | ||
+ | | 1.427 | ||
+ | |- | ||
+ | |WF yellow | ||
+ | | 4.22 / 238.204 | ||
+ | | 10.47 / 361.287 | ||
+ | | 1.421 | ||
+ | |- | ||
+ | |WF green | ||
+ | | | ||
+ | | | ||
+ | | | ||
+ | |- | ||
+ | |WF blue | ||
+ | | 5.17 / 238.863 | ||
+ | | 12.58 / 214.439 | ||
+ | | 1.322 | ||
+ | |- | ||
+ | |WF pink | ||
+ | | 4.78 / 261.382 | ||
+ | | 10.89 / 361.287 | ||
+ | | 1.486 | ||
+ | |- | ||
+ | |WF purple | ||
+ | | 4.52 / 261.382 | ||
+ | | 11.52 / 361.287 | ||
+ | | 1.205 | ||
+ | |- | ||
+ | |WF WT | ||
+ | | | ||
+ | | | ||
+ | | 1.18 | ||
+ | |- | ||
+ | |WF Fet4 | ||
+ | | | ||
+ | | | ||
+ | | 1.4 | ||
+ | |} | ||
+ | |||
+ | ===Cytotoxicity data=== | ||
+ | [[https://www.dropbox.com/sh/rjhotqgpy2v37se/AACOnsHoEBZMNvQ7DRd2D5Yma?dl=0 TR section]] | ||
+ | [[https://www.dropbox.com/s/u0ydemw0472ekz2/WF.xlsx?dl=0 WF section]] | ||
+ | |||
+ | 1) Untransformed No Cadmium OD - 1.5 | ||
+ | 2) Untransformed Cadmium OD - 1.4 | ||
+ | 3) FET4 No Cadmium OD - 1.44 | ||
+ | 4) FET4 Cadmium OD - 1.44 | ||
+ | 5) Purple No Cadmium 1.733 | ||
+ | 6) Purple Cadmium 1.778 |
Latest revision as of 17:59, 12 May 2023
Contents
Module 1: Drug discovery
SDS-PAGE
T/R
Red Team: Media:TR Red S23M1D3.jpg
Orange Team: Media:TR Orange S23M1D3.jpg
Yellow Team: Media:TR Yellow S23M1D3.jpg
Green Team: Media:TR Green S23M1D3.jpg
Blue Team: Media:TR Blue S23M1D3.jpg
Pink Team: Media:TR Pink S23M1D3.jpg
Purple Team: Media:TR Purple S23M1D3.jpg
W/F
Dropbox Link : https://www.dropbox.com/sh/1p3sfs2487pkwr0/AAAAA-W0W6vPcXcfcp2nV2TPa?dl=0
BCA
W/F
Western Blot
W/F
Dropbox Link: https://www.dropbox.com/scl/fo/xlv4yz02oq2pfhka7mcmt/h?dl=0&rlkey=x1g0jqms054n0ycy3s2atbzrf
SMM
Module 2: Protein engineering
Mutagenesis
T/R
Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
TR red | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
TR orange | Y276C | TTGGCAGATGtgtATTAACACTG | GCGTCACTGAACTTTTTC |
TR yellow | Y276C | GGCAGATGTAcATTAACACTG | AAGCGTCACTGAACTTTTTC |
TR green | Y352C | GATTGATTGGtgtGCTGATATTATTG | ATCTTTCTCCCTGTCGAC |
TR blue | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
TR pink | D354C | TTGGTATGCTtgtATTATTGGTACTGG | TCAATCATCTTTCTCCCTG |
TR purple | Y276C | TGGCAGATGTgTATTAACACTG | AGCGTCACTGAACTTTTTC |
T/R Sequencing [here]
W/F
Team | Mutation (eg. S301L) | Forward primer | Reverse primer |
WF red | V281C | TAACACTGCAtgTGCAGTTTCCCTTC | ATATACATCTGCCAAGCG |
WF orange | I349C | GAGAAAGATGtgTGATTGGTATGCTGATATTATTG | CCTGTCGACCTTTTTTTAG |
WF yellow | R405C | TTTCGTTTTGtgtGAAGTGTACTTCCG | CCGTCCAAAAACCCAATTAAAC |
WF green | D271C | AAAGTTCAGTTGCGCTTGGCAGATGTATATTAACACTGCAGTTGC (AAAGTTCAGTtgcGCTTGGCAGATG) | TTCAATCTTGGATTACTACCTGTAGTTTCTCCGGTGTAAGGTGGA (TTCAATCTTGGATTACTACCTG) |
WF blue | Y408C | ||
WF pink | D400C | ATTGGGTTTTTGtgCGGTTTCGTTTTG (TGGGTTTTTGtgcGGTTTCGTTTTG) | TAAACCTGTGTATGTACCGATAATCAACCACC (ATTAAACCTGTGTATGTACC) |
WF purple | D350C | AAAGATGATTtgtTGGTATGCTGATATTATTGGTAC | CTCCCTGTCGACCTTTTTTTAG |
WF Sequencing here
Immunofluorescence Data
Pilot Immunofluorescence Data [[1]]
ICP-OES data
T/R
Raw ICP-OES data [here]
Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
TR red | 2.62 ppm / 240.489 nm | 7.58 ppm / 361.287 nm | 1.889 |
TR orange | 4.29 ppm / 261.382 nm | 11.16 ppm / 326.105 nm | 1.566 |
TR yellow | 3.70 ppm / 261.382 nm | 11.35 ppm / 346.766 nm | 1.529 |
TR green | 4.58 ppm / 238.863 nm | 11.50 ppm / 479.991 nm | 1.495 |
TR blue | 4.66 ppm / 259.837 nm | 11.52 ppm /479.999 nm | 1.546 |
TR pink | 4.03ppm / 238.863nm | 10.52ppm / 232.929nm | 1.581 |
TR purple | 4.19 ppm / 238.204 nm | 10.90 ppm / 223.986 nm | 1.458 |
TR WT | 1 | ||
TR Fet4 | 1.28 |
W/F
Raw ICP-OES data [here]
Team | Fe (ppm)/ Wavelength | Cd (ppm)/ Wavelength | O.D. |
WF red | 4.09 / 238.863 | 10.99 /213.439 | 1.486 |
WF orange | 3.73 / 238.204 | 11.63 / 226.502 | 1.427 |
WF yellow | 4.22 / 238.204 | 10.47 / 361.287 | 1.421 |
WF green | |||
WF blue | 5.17 / 238.863 | 12.58 / 214.439 | 1.322 |
WF pink | 4.78 / 261.382 | 10.89 / 361.287 | 1.486 |
WF purple | 4.52 / 261.382 | 11.52 / 361.287 | 1.205 |
WF WT | 1.18 | ||
WF Fet4 | 1.4 |
Cytotoxicity data
[TR section] [WF section]
1) Untransformed No Cadmium OD - 1.5 2) Untransformed Cadmium OD - 1.4 3) FET4 No Cadmium OD - 1.44 4) FET4 Cadmium OD - 1.44 5) Purple No Cadmium 1.733
6) Purple Cadmium 1.778