Difference between revisions of "Optics Bootcamp"
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==Overview== | ==Overview== | ||
This lab exercise will introduce you to some of the optical components that you will use in the microscopy lab. You will build an apparatus that includes an LED illuminator, an object with precisely spaced markings, a lens, and a CCD camera. You will place the object at several distances from the lens and measure the corresponding image distance. Using the MATLAB Image Acquisition Tool, you will record images and use them to measure magnification. Finally, you will compare your measurements to the values given by the lens makers' formula that was derived in class. | This lab exercise will introduce you to some of the optical components that you will use in the microscopy lab. You will build an apparatus that includes an LED illuminator, an object with precisely spaced markings, a lens, and a CCD camera. You will place the object at several distances from the lens and measure the corresponding image distance. Using the MATLAB Image Acquisition Tool, you will record images and use them to measure magnification. Finally, you will compare your measurements to the values given by the lens makers' formula that was derived in class. | ||
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===Orientation=== | ===Orientation=== | ||
[[Image:20.390 Lab Map.png|Map of the 20.309 lab.]] | [[Image:20.390 Lab Map.png|Map of the 20.309 lab.]] | ||
− | There are more than 15,000 bits and pieces in the 20.309 lab. Many of them look alike. It's important to learn your way around the lab so you don't waste a lot of time looking for things. Take a walk around the whole lab and check things out. Spend at least five minutes poking around. Read the time machine poster | + | There are more than 15,000 bits and pieces in the 20.309 lab. Many of them look alike. It's important to learn your way around the lab so you don't waste a lot of time looking for things. The floor plan above shows the layout of the lab. Take a walk around the whole lab and check things out. Spend at least five minutes poking around. Read the time machine poster between the cable rake and the Nikon microscope. Discover the stunning Studley tool chest poster near the east parts cabinet. See! Wasn't that a worthwhile journey? |
Now go ahead and gather all the parts you will need. | Now go ahead and gather all the parts you will need. | ||
− | == | + | ==Gather materials== |
− | + | [[Image: 140729_OpticsBootcamp_02.jpg|thumb|right]] | |
− | + | The first step is to gather the materials required to build the lens measuring apparatus. The lists below include part numbers and descriptive names of all the components in the apparatus. It is likely that you will find some of the terms not-all-that-self-explanatory. Most of the parts are manufactured by a company called ThorLabs. If you have a question about any of the components, the [http://www.thorlabs.com ThorLabs website] can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such an thing might look like, try googling the term: "thorlabs SPW602". You will find your virtual self just a click or two away from [http://www.thorlabs.com/thorproduct.cfm?partnumber=SPW602 a handsome photo and detailed specifications]. | |
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+ | ===Optomechanics=== | ||
+ | These components are located in plastic bins on top of the center parts cabinet: | ||
* 1 x RLA1800 dovetail optical rails | * 1 x RLA1800 dovetail optical rails | ||
* 4 x RC1 rail carriers | * 4 x RC1 rail carriers | ||
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* 1 x SM1L10 lens tube | * 1 x SM1L10 lens tube | ||
+ | * 1 x SM1RC lens tube slip ring | ||
+ | * 1 x CP02 cage plate | ||
* 1 x LCP01 cage plate (looks like an "O" in a square) | * 1 x LCP01 cage plate (looks like an "O" in a square) | ||
* 1 x LCP02 cage plate adapter (looks like an "X") | * 1 x LCP02 cage plate adapter (looks like an "X") | ||
+ | * 2 x SM2RR retaining rings | ||
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+ | These components are located on top of the south parts cabinet | ||
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* 4 x ER1 cage assembly rod | * 4 x ER1 cage assembly rod | ||
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* 6 x SM1RR retaining rings | * 6 x SM1RR retaining rings | ||
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===Screws and posts=== | ===Screws and posts=== | ||
− | + | Screws and posts are located on top of the west parts cabinet. | |
− | * 4 x PH2 | + | |
+ | * 4 x PH2 post holders | ||
* 4 x TR2 optical posts | * 4 x TR2 optical posts | ||
* 4 x 8-32 set screws | * 4 x 8-32 set screws | ||
+ | * 6 x 1/4-20 x 5/16" cap screws | ||
* 1 x 1/4-20 set screw | * 1 x 1/4-20 set screw | ||
* 8 x 4-40 set screws | * 8 x 4-40 set screws | ||
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==Build the apparatus== | ==Build the apparatus== | ||
+ | ===Optical rails=== | ||
+ | Optical rails are useful for arranging components in a line that require variable separation. Sliding clamps sit on the rail. The clamps have a thumbscrew that locks them in position. Rails and clamps are located on top of the center parts cabinet. Here is a list of the optical rails and clamps you will need: | ||
{|class="wikitable" | {|class="wikitable" |
Revision as of 16:03, 2 September 2014
Overview
This lab exercise will introduce you to some of the optical components that you will use in the microscopy lab. You will build an apparatus that includes an LED illuminator, an object with precisely spaced markings, a lens, and a CCD camera. You will place the object at several distances from the lens and measure the corresponding image distance. Using the MATLAB Image Acquisition Tool, you will record images and use them to measure magnification. Finally, you will compare your measurements to the values given by the lens makers' formula that was derived in class.
Orientation
There are more than 15,000 bits and pieces in the 20.309 lab. Many of them look alike. It's important to learn your way around the lab so you don't waste a lot of time looking for things. The floor plan above shows the layout of the lab. Take a walk around the whole lab and check things out. Spend at least five minutes poking around. Read the time machine poster between the cable rake and the Nikon microscope. Discover the stunning Studley tool chest poster near the east parts cabinet. See! Wasn't that a worthwhile journey?
Now go ahead and gather all the parts you will need.
Gather materials
The first step is to gather the materials required to build the lens measuring apparatus. The lists below include part numbers and descriptive names of all the components in the apparatus. It is likely that you will find some of the terms not-all-that-self-explanatory. Most of the parts are manufactured by a company called ThorLabs. If you have a question about any of the components, the ThorLabs website can be very helpful. For example, if the procedure calls for an SPW602 spanner wrench and you have no idea what such an thing might look like, try googling the term: "thorlabs SPW602". You will find your virtual self just a click or two away from a handsome photo and detailed specifications.
Optomechanics
These components are located in plastic bins on top of the center parts cabinet:
- 1 x RLA1800 dovetail optical rails
- 4 x RC1 rail carriers
- 1 x SM1L10 lens tube
- 1 x SM1RC lens tube slip ring
- 1 x CP02 cage plate
- 1 x LCP01 cage plate (looks like an "O" in a square)
- 1 x LCP02 cage plate adapter (looks like an "X")
- 2 x SM2RR retaining rings
These components are located on top of the south parts cabinet
- 4 x ER1 cage assembly rod
- 6 x SM1RR retaining rings
Screws and posts
Screws and posts are located on top of the west parts cabinet.
- 4 x PH2 post holders
- 4 x TR2 optical posts
- 4 x 8-32 set screws
- 6 x 1/4-20 x 5/16" cap screws
- 1 x 1/4-20 set screw
- 8 x 4-40 set screws
Optics
- 1 x plano-convex f = 25 mm lens
- 1 x LB1811 biconvex f = 35 mm lens
Object
- 1 x R1DS1N 1951 USAF test target
Optoelectronics
- 1 x red LED
Tools
Here is a list of the tools you will need:
- 1 x SPW602 spanner wrench
- 1 x SPW801 adjustable spanner wrench
- 1 x 3/16 balldriver for 1/4-20 cap screws
- 1 x 9/64 balldriver
- 1 x 0.050" hex balldriver for 4-40 set screws
Things that should already be (and stay at) your lab station
- 1 x Manta CCD camera
- 1 x Calrad 45-601 power adapter for CCD
- 1 x ethernet cable connected to the lab station computer
Build the apparatus
Optical rails
Optical rails are useful for arranging components in a line that require variable separation. Sliding clamps sit on the rail. The clamps have a thumbscrew that locks them in position. Rails and clamps are located on top of the center parts cabinet. Here is a list of the optical rails and clamps you will need:
Visualize, capture, and save images in Matlab
Examine images in Matlab
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Plot and discuss your results
- Repeat these measurements of $ S_o $, $ S_i $, $ h_o $, and $ h_i $ for several values of $ S_o $.
- Plot $ {1 \over S_i} $ as a function of $ = {1 \over f} - {1 \over S_o} $.
- Plot $ h_i $ as a function of $ h_o $.
- What sources of error affect your measurements?
- What is the uncertainty of your magnification measurement?
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients