Difference between revisions of "Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)"
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==IPC mutants (and primers) chosen by teams== | ==IPC mutants (and primers) chosen by teams== | ||
<font color = #FF0000>'''T/R RED'''</font color> | <font color = #FF0000>'''T/R RED'''</font color> | ||
− | *mutation: | + | *mutation: D132H |
*forward primer: | *forward primer: | ||
*reverse primer: | *reverse primer: | ||
*restriction enzyme: | *restriction enzyme: | ||
+ | <br><font color = #FF0000>'''W/F RED'''</font color> | ||
+ | *mutation: D57H | ||
+ | *forward primer: GCC GGC AGT CGA TGC TCA TGG CAA TGG AA | ||
+ | *reverse primer: TC ATT GAT CAT ATC CTG CAA TTC | ||
+ | *restriction enzyme: NaeI - GCCGGC | ||
<br><font color = #FF6600>'''T/R ORANGE'''</font color> | <br><font color = #FF6600>'''T/R ORANGE'''</font color> | ||
− | *mutation: | + | *mutation: T78P |
*forward primer: | *forward primer: | ||
*reverse primer: | *reverse primer: | ||
− | *restriction enzyme: | + | *restriction enzyme: |
+ | <br><font color = #FFFF00>'''W/F YELLOW'''</font color> | ||
+ | *mutation: D21A | ||
+ | *forward primer: 5' GCC GGC ATT CGA CAA GGC TGG GGA CGG CA | ||
+ | *reverse primer: 5' AAT GAG AAG GCT TCT TTG AAC TCT GC | ||
+ | *restriction enzyme: NaeI - GCCGGC | ||
<br><font color = #00FF00>'''T/R GREEN'''</font color> | <br><font color = #00FF00>'''T/R GREEN'''</font color> | ||
− | *mutation: | + | *mutation: D57H |
*forward primer: | *forward primer: | ||
*reverse primer: | *reverse primer: | ||
*restriction enzyme: | *restriction enzyme: | ||
+ | <br><font color = #00FF00>'''W/F GREEN'''</font color> | ||
+ | *mutation: T78P | ||
+ | *forward primer: 5' CCA GGG AAT GAA GGA CCC AGA CAG CGA AG 3' | ||
+ | *reverse primer: 5' TTT CTA GCC ATC ATA GTA AGA AAT TCA GGA AAG TAA ATC GTT CC 3' | ||
+ | *restriction enzyme: StyI - C/CWWGG | ||
<br><font color = #0000FF>'''T/R BLUE'''</font color> | <br><font color = #0000FF>'''T/R BLUE'''</font color> | ||
− | *mutation: | + | *mutation: D132H |
− | *forward primer: | + | *forward primer: 5' TAT CGA TGG TCA TGG CCA AGT AAA C 3' |
− | *reverse primer: | + | *reverse primer: 5’ TCT GCT TCC CTT ATC ATT TC 3’ |
− | *restriction enzyme: | + | *restriction enzyme: None (no flap included) |
+ | <br><font color = #0000FF>'''W/F BLUE'''</font color> | ||
+ | *mutation: E30K | ||
+ | *forward primer: 5' CCA GGG CAC CAC AAA GAA ACT TGG CAC CG 3' | ||
+ | *reverse primer: 5' ATGGTGCCGTCCCCATCC 3' | ||
+ | *restriction enzyme: StyI-HF - C/CWWGG | ||
<br><font color = #FF66CC>'''T/R PINK'''</font color> | <br><font color = #FF66CC>'''T/R PINK'''</font color> | ||
− | *mutation: | + | *mutation: D132H |
− | *forward primer: | + | *forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3' |
− | *reverse primer: | + | *reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3' |
− | *restriction enzyme: | + | *restriction enzyme: NaeI (GCC / GGC) |
+ | <br><font color = #FF66CC>'''W/F PINK'''</font color> | ||
+ | *mutation: D132H | ||
+ | *forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3' | ||
+ | *reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3' | ||
+ | *restriction enzyme: NaeI (GCC / GGC) |
Latest revision as of 18:51, 16 October 2015
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F RED
- mutation: D57H
- forward primer: GCC GGC AGT CGA TGC TCA TGG CAA TGG AA
- reverse primer: TC ATT GAT CAT ATC CTG CAA TTC
- restriction enzyme: NaeI - GCCGGC
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
W/F YELLOW
- mutation: D21A
- forward primer: 5' GCC GGC ATT CGA CAA GGC TGG GGA CGG CA
- reverse primer: 5' AAT GAG AAG GCT TCT TTG AAC TCT GC
- restriction enzyme: NaeI - GCCGGC
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F GREEN
- mutation: T78P
- forward primer: 5' CCA GGG AAT GAA GGA CCC AGA CAG CGA AG 3'
- reverse primer: 5' TTT CTA GCC ATC ATA GTA AGA AAT TCA GGA AAG TAA ATC GTT CC 3'
- restriction enzyme: StyI - C/CWWGG
T/R BLUE
- mutation: D132H
- forward primer: 5' TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5’ TCT GCT TCC CTT ATC ATT TC 3’
- restriction enzyme: None (no flap included)
W/F BLUE
- mutation: E30K
- forward primer: 5' CCA GGG CAC CAC AAA GAA ACT TGG CAC CG 3'
- reverse primer: 5' ATGGTGCCGTCCCCATCC 3'
- restriction enzyme: StyI-HF - C/CWWGG
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)
W/F PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)