ThorLabs OTKB optical trapping kit

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Thorlabs optical trapping kit

OTKB construction

Modifications to ThorLabs suggested layout

Alignment procedure

Comparison of OTKB and 20.309 optical trap operating characteristics

Position calibration

The position calibrations determine the sensitivity of the quadrant photodiode detector to changes in position of a bead. Accurate quantitative results depend on precise position calibration.

Methodology

Results

ThorLabs OTKB 20.309
X position TBD X-axis Position Calibration
Y position TBD Y-axis Position Calibration

Trap stiffness

The force calibrations determine the stiffness of the trap. The stiffness of the trap characterizes the forces exerted by it. Three different methods were used in order to determine the stiffness of each trap: Equipartition, noise PSD roll-off, and Stokes drag.

Methodology

Results

Equipartition
ThorLabs OTKB 20.309
X and Y axes TBD X and Y axis Force Calibration