Difference between revisions of "Talk:20.109(S17):Module 2"

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==Instructor's Western Blot==
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[[Image:Instructor_TR_031617.jpeg |thumb|left|400px| Instructor's Western Blot]]
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<div style="padding: 10px; width: 790px; border: 5px solid #6633CC;">
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==References from Prof. Samson Lecture 8==
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*[[Media:Thermo BRCA2 cell line .pdf| A syngeneic variance library for functional annotation of human variation: application to ''BRCA2''.]]
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*[[Media:Adachi et al BioChem 2003.pdf| Hypersensitivity of Nonhomologous DNA End-joining Mutants to VP-16 and ICRF-193.]]
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*[[Media:Engineered BRCA2mutations Invitrogen.pdf| Compound screening in BRCA2 (–/–) GeneArt Engineered Cell Model.]]
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*[[Media:Lin et al PLOS 2009.pdf| p63 and p73 Transcriptionally Regulate Genes Involved in DNA Repair.]]
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*[[Media:Nagel et al PNAS 2014.pdf| Multiplexed DNA repair assays for multiple lesions and multiple doses via transcription inhibition and transcriptional mutagenesis.]]
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*[[Media:Treszezamsky et al CanRes 2007.pdf| BRCA1- and BRCA2-Deficient Cells Are Sensitive to Etoposide-Induced DNA Double-Strand Breaks via Topoisomerase II.]]
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==quantitative PCR data==
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[[Media:20.109 Spring 2017, M2 qPCR results v2.xlsx|qPCR data ]]
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==CellTiter Glo luminescence cell viability assay==
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[[Media: CellTiterGlo_TR170321.xlsx| TR data]]<br>
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[[Media: CellTiterGlo_WF170322.xlsx| WF data]]
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==Western blot images==
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===Instructor's Western Blot===
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[[Image:Instructor_TR_031617.jpg |thumb|left|100px| Instructor's Western Blot]]
  
  
 
<br style="clear:both;"/>
 
<br style="clear:both;"/>
  
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==T/R Western Blots==
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===T/R Western Blots===
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[[Image:|thumb|left|400px| TR Purple and Blue Team]]
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[[Image:Silver_TR_031617.jpg|thumb|left|100px| TR silver]]
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[[Image:|thumb|left|400px| TR Pink]]
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[[Image:Pink_TR_031617.jpg|thumb|left|100px| TR Pink]]
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[[Image: |thumb|left|400px| TR Green]]
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[[Image: Green_TR_031617.jpg|thumb|left|100px| TR Green]]
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[[Image: |thumb|left|400px| TR Yellow]]
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[[Image: Yellow_TR_031617.jpg|thumb|left|100px| TR Yellow]]
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[[Image: |thumb|left|400px| TR Orange]]
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[[Image: Red_TR_031617.jpg|thumb|left|100px| TR Red]]
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[[Image: |thumb|left|400px| TR Red]]
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[[Image: Blue_TR_031617.jpg|thumb|left|100px| TR Blue]]
  
 
<br style="clear:both;"/>
 
<br style="clear:both;"/>
  
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==W/F Western Blots==
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===W/F Western Blots===
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[[Image:Sp17_Mod2_WFgel1_700.jpg |thumb|left|400px| Red, Yellow, Green samples]]
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[[Image:Sp17_Mod2_WFgel2_700.jpg |thumb|left|400px| Blue, Pink Purple samples]]
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<br style="clear:both;"/>

Latest revision as of 19:41, 13 April 2017

20.109(S17): Laboratory Fundamentals of Biological Engineering

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Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

References from Prof. Samson Lecture 8

quantitative PCR data

qPCR data

CellTiter Glo luminescence cell viability assay

TR data
WF data

Western blot images

Instructor's Western Blot

Instructor's Western Blot



T/R Western Blots

TR silver
TR Pink
TR Green
TR Yellow
TR Red
TR Blue


W/F Western Blots

Red, Yellow, Green samples
Blue, Pink Purple samples

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