Talk:20.109(S16):Purify protein (Day6)

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OD562 absorbance measurements for protein concentration calculations

During the 1 hour incubation of Part4A use excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data.

T/R red team

name OD562
standards
A
B
C
D
E
F
G
H
I
inverse pericam (IPC)
wild-type
mutant