Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"
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===OD562 absorbance measurements for protein concentration calculations=== | ===OD562 absorbance measurements for protein concentration calculations=== | ||
| − | During the 1 hour incubation of Part4A use excel to prepare a graph of absorbance versus BSA concentration using | + | During the 1 hour incubation of Part4A use excel to prepare a graph of absorbance versus BSA concentration using the sample data [[Media:SampleData_microBCA.xlsx| '''here'''.]] You will need to use the trendline equation to calculate the protein concentration of your example elution data. |
====T/R red team==== | ====T/R red team==== | ||
{| border="1" | {| border="1" | ||
Revision as of 17:48, 22 February 2016
OD562 absorbance measurements for protein concentration calculations
During the 1 hour incubation of Part4A use excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data.
T/R red team
| name | OD562 |
| standards | |
| A | |
| B | |
| C | |
| D | |
| E | |
| F | |
| G | |
| H | |
| I | |
| inverse pericam (IPC) | |
| wild-type | |
| mutant | |
