Difference between revisions of "Talk:20.109(S16):Design mutation primers (Day2)"
From Course Wiki
m (→Primers for SDM) |
|||
| Line 44: | Line 44: | ||
|- | |- | ||
|<font color = #FF0000>WF red</font color> | |<font color = #FF0000>WF red</font color> | ||
| − | |5' | + | |5' CAAGGATGGGcACGGCACCAT 3' |
| − | |5' | + | |5' TCGAATAATGAGAAGGCTTCTTTG 3' |
| | | | ||
|- | |- | ||
Revision as of 03:23, 11 February 2016
Primers for SDM
| Team | Forward primer | Reverse primer | Mutation (X#Z in CaM) | |
| TR red | 5' CACCACAAAGagACTTGGCACCGTTATG 3' | 5' ATGGTGCCGTCCCCATCC 3' | E30R | |
| TR orange | 5' ATTCGACAAGcatGGGGACGGCA 3' | 5' AATGAGAAGGCTTCTTTGAACTCTG 3' | D21H | |
| TR yellow | CACCACAAAGaAACTTGGCACCG | ATGGTGCCGTCCCCATCC | ||
| TR green | 5' TTCGACAAGGgTGGGGACGGC 3' | 5' TAATGAGAAGGCTTCTTTGAACTCTGC 3' | D62G | |
| TR blue | 5' CAAGGATGGGaACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D67N | |
| TR pink | 5' GTT GAT GAA ATG ATA AGG GAA cCA GAT ATC GAT GGT GAT GGC C 3' | 5' TTC TTC ATC TGT TAA CTT CTC CCC GAG GTT TGT CAT 3' | A127P | |
| TR purple | 5' TTTTGACAAGcATGGGAACGGC 3' | 5' ACACGGAATGCTTCTCGG 3' | D94H | |
| WF red | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | ||
| WF orange | 5' insert here 3' | 5' insert here 3' | ||
| WF blue | 5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | 5' CCATCAGCATCGACTTCATTG 3' | ||
| WF pink | 5' CGT TCC ATT GCC ATC AGC ATC GAC TTC ATT GAT CAT ATC 3' | 5' CTG CAA TTC TGC TTC CGT TGG GTT TTG TCC AAG CGA CC 3' | ||
| WF purple | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' |
