Difference between revisions of "Talk:20.109(S16):Design mutation primers (Day2)"
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MAXINE JONAS (Talk | contribs) (→Primers) |
(→Primers for SDM) |
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|'''Forward primer''' | |'''Forward primer''' | ||
|'''Reverse primer''' | |'''Reverse primer''' | ||
| − | |-- | + | |'''Mutation (X#Z in CaM)''' |
| − | |TR red | + | |- |
| − | |5' | + | |<font color = #FF0000>TR red</font color> |
| − | |5' | + | |5' CACCACAAAGagACTTGGCACCGTTATG 3' |
| + | |5' ATGGTGCCGTCCCCATCC 3' | ||
| + | | E30R | ||
| + | |- | ||
| + | |<font color = #FF9900>TR orange</font color> | ||
| + | |5' ATTCGACAAGcatGGGGACGGCA 3' | ||
| + | |5' AATGAGAAGGCTTCTTTGAACTCTG 3' | ||
| + | | D21H | ||
| + | |- | ||
| + | |<font color = #FFFF00>TR yellow</font color> | ||
| + | |CACCACAAAGaAACTTGGCACCG | ||
| + | |ATGGTGCCGTCCCCATCC | ||
| + | | E30K | ||
| + | |- | ||
| + | |<font color = #00FF00>TR green</font color> | ||
| + | |5' TTCGACAAGGgTGGGGACGGC 3' | ||
| + | |5' TAATGAGAAGGCTTCTTTGAACTCTGC 3' | ||
| + | | D62G | ||
| + | | | ||
| + | |- | ||
| + | |<font color = #0000FF>TR blue</font color> | ||
| + | |5' CAAGGATGGGaACGGCACCAT 3' | ||
| + | |5' TCGAATAATGAGAAGGCTTCTTTG 3' | ||
| + | | D23N | ||
| + | |- | ||
| + | |<font color = #FF99CC>TR pink</font color> | ||
| + | |5' GTT GAT GAA ATG ATA AGG GAA cCA GAT ATC GAT GGT GAT GGC C 3' | ||
| + | |5' TTC TTC ATC TGT TAA CTT CTC CCC GAG GTT TGT CAT 3' | ||
| + | |A127P | ||
| + | |- | ||
| + | |<font color = #CC00FF>TR purple</font color> | ||
| + | |5' TTTTGACAAGcATGGGAACGGC 3' | ||
| + | |5' ACACGGAATGCTTCTCGG 3' | ||
| + | | D94H | ||
| + | |- | ||
| + | |<font color = #FF0000>WF red</font color> | ||
| + | |5' CAAGGATGGGcACGGCACCAT 3' | ||
| + | |5' TCGAATAATGAGAAGGCTTCTTTG 3' | ||
| + | | D23H | ||
| + | |- | ||
| + | |<font color = #FF9900>WF orange</font color> | ||
| + | |5'CCGT GTT TTT GAC gAG GAT GGG AAC 3' | ||
| + | |5' AAT GCT TCT CGG ATT TCC TCT TCG C 3' | ||
| + | |K93E | ||
| + | |- | ||
| + | |<font color = #0000FF>WF blue</font color> | ||
| + | |5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | ||
| + | |5' CCATCAGCATCGACTTCATTG 3' | ||
| + | |I62D | ||
| + | |- | ||
| + | |<font color = #FF99CC>WF pink</font color> | ||
| + | |5' AAT GAA GTC GCT GCT GAT GGC 3' | ||
| + | |5' GAT CAT ATC CTG CAA TTC TG 3' | ||
| + | |D55A | ||
| + | |- | ||
| + | |<font color = #CC00FF>WF purple</font color> | ||
| + | |5' CAAGGATGGGcACGGCACCAT 3' | ||
| + | |5' TCGAATAATGAGAAGGCTTCTTTG 3' | ||
| + | |D23H | ||
|} | |} | ||
Latest revision as of 15:20, 25 February 2016
Primers for SDM
| Team | Forward primer | Reverse primer | Mutation (X#Z in CaM) | |
| TR red | 5' CACCACAAAGagACTTGGCACCGTTATG 3' | 5' ATGGTGCCGTCCCCATCC 3' | E30R | |
| TR orange | 5' ATTCGACAAGcatGGGGACGGCA 3' | 5' AATGAGAAGGCTTCTTTGAACTCTG 3' | D21H | |
| TR yellow | CACCACAAAGaAACTTGGCACCG | ATGGTGCCGTCCCCATCC | E30K | |
| TR green | 5' TTCGACAAGGgTGGGGACGGC 3' | 5' TAATGAGAAGGCTTCTTTGAACTCTGC 3' | D62G | |
| TR blue | 5' CAAGGATGGGaACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23N | |
| TR pink | 5' GTT GAT GAA ATG ATA AGG GAA cCA GAT ATC GAT GGT GAT GGC C 3' | 5' TTC TTC ATC TGT TAA CTT CTC CCC GAG GTT TGT CAT 3' | A127P | |
| TR purple | 5' TTTTGACAAGcATGGGAACGGC 3' | 5' ACACGGAATGCTTCTCGG 3' | D94H | |
| WF red | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23H | |
| WF orange | 5'CCGT GTT TTT GAC gAG GAT GGG AAC 3' | 5' AAT GCT TCT CGG ATT TCC TCT TCG C 3' | K93E | |
| WF blue | 5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | 5' CCATCAGCATCGACTTCATTG 3' | I62D | |
| WF pink | 5' AAT GAA GTC GCT GCT GAT GGC 3' | 5' GAT CAT ATC CTG CAA TTC TG 3' | D55A | |
| WF purple | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23H |
