Difference between revisions of "Spring 2012:LFM Report"
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Vincent Lee & Leanna Morinishi | Vincent Lee & Leanna Morinishi | ||
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− | + | = Light Field Microscope = | |
− | = Introduction and Motivation = | + | == Introduction and Motivation == |
A light field microscope (LFM) is capable of producing a 3-dimensional rendering of a sample using information from a single image. The addition of a microlens array, a grid of lenses with diameters on the microscale, to a traditional illumination microscope grants this capability. Here we propose integrating a Lytro™ camera into a basic wide field and epifluorescence microscope, associated code and recommended experiments for use in a teaching undergraduate laboratory. | A light field microscope (LFM) is capable of producing a 3-dimensional rendering of a sample using information from a single image. The addition of a microlens array, a grid of lenses with diameters on the microscale, to a traditional illumination microscope grants this capability. Here we propose integrating a Lytro™ camera into a basic wide field and epifluorescence microscope, associated code and recommended experiments for use in a teaching undergraduate laboratory. | ||
− | = Microscope Design = | + | == Microscope Design == |
− | = Reverse engineering the Lytro™ images = | + | == Reverse engineering the Lytro™ images = = |
− | = Dealing with the hexagonal microarray = | + | == Dealing with the hexagonal microarray == |
− | = Acknowledgments = | + | == Acknowledgments == |
# Nirav Patel, for reverse engineering the Lytro image and lfpsplitter. | # Nirav Patel, for reverse engineering the Lytro image and lfpsplitter. | ||
# Frank Warmerdam, Andrey Kiselev, Bob Friesenhahn, Joris Van Damme and Lee Howard for raw2tiff tools. | # Frank Warmerdam, Andrey Kiselev, Bob Friesenhahn, Joris Van Damme and Lee Howard for raw2tiff tools. |
Revision as of 06:48, 17 May 2012
Contents
Design and Construction of a Light Field Microscope
Vincent Lee & Leanna Morinishi
Light Field Microscope
Introduction and Motivation
A light field microscope (LFM) is capable of producing a 3-dimensional rendering of a sample using information from a single image. The addition of a microlens array, a grid of lenses with diameters on the microscale, to a traditional illumination microscope grants this capability. Here we propose integrating a Lytro™ camera into a basic wide field and epifluorescence microscope, associated code and recommended experiments for use in a teaching undergraduate laboratory.
Microscope Design
= Reverse engineering the Lytro™ images =
Dealing with the hexagonal microarray
Acknowledgments
- Nirav Patel, for reverse engineering the Lytro image and lfpsplitter.
- Frank Warmerdam, Andrey Kiselev, Bob Friesenhahn, Joris Van Damme and Lee Howard for raw2tiff tools.