Design and Construction of a Light Field Microscope <h3> <h3> Vincent Lee, Leanna Morinishi <h3>

Introduction and Motivation

A light field microscope (LFM) is capable of producing a 3-dimensional rendering of a sample using information from a single image. The addition of a microlens array, a grid of lenses with diameters on the microscale, to a traditional illumination microscope grants this capability. Here we propose integrating a Lytro™ camera into a basic wide field and epifluorescence microscope, associated code and recommended experiments for use in a teaching undergraduate laboratory.

Microscope Design

Reverse engineering the Lytro™ images

Dealing with the hexagonal microarray

Acknowledgments

1. Nirav Patel, for reverse engineering the Lytro image and lfpsplitter.
2. Frank Warmerdam, Andrey Kiselev, Bob Friesenhahn, Joris Van Damme and Lee Howard for raw2tiff tools.

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