Difference between revisions of "Spring 11:Directed Evolution/Week of 4-18-2011 Status"
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* Continued coding image analysis / segmentation code. | * Continued coding image analysis / segmentation code. | ||
* Fabricated Microfluidics using resources from Kamm lab and Voldemann lab. | * Fabricated Microfluidics using resources from Kamm lab and Voldemann lab. | ||
| + | ** PDMS poured & cured Monday afternoon | ||
* Moved microscope setup to larger breadboard. | * Moved microscope setup to larger breadboard. | ||
Revision as of 20:59, 18 April 2011
Weekly Progress
Weekend/Monday
- Finalized parts list.
- Continued coding image analysis / segmentation code.
- Fabricated Microfluidics using resources from Kamm lab and Voldemann lab.
- PDMS poured & cured Monday afternoon
- Moved microscope setup to larger breadboard.
Monday Evening
- Will start a culture of I13521 from freezer stock. ~12 hours of grow time should be sufficient. Also, potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the fliC273 gene.
Notes
Biology
- David Liu has agreed to give us MP-QUR, pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid.
- The Elowitz Repressilator can apparently be found on Add-Gene, will need to either find or figure out a good testing rig to put it in.
- Cory has I13521 and I13522, although both are in pSB1A3, which might prove problematic if the KAF95 fliC is also on a pBR322 derived plasmid. Current backup is J04450 which can be found in pSB1C3 and would allow for a co-transformant.
