Difference between revisions of "Spring 11:Directed Evolution/Week of 4-18-2011 Status"
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(Created page with "==Weekend/Monday== Initial chambers for testing cells & fluorescent beads * Finalized parts list. * Continued coding image analysis / segmentation...") |
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| + | =Weekly Progress= | ||
==Weekend/Monday== | ==Weekend/Monday== | ||
[[Image:DE_Fluidics.png|thumb|Initial chambers for testing cells & fluorescent beads]] | [[Image:DE_Fluidics.png|thumb|Initial chambers for testing cells & fluorescent beads]] | ||
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* Moved microscope setup to larger breadboard. | * Moved microscope setup to larger breadboard. | ||
| + | ==Monday Evening== | ||
| + | * Will start a culture of I13521 from freezer stock. ~12 hours of grow time should be sufficient. Also, potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the ''fliC273'' gene. | ||
| − | + | ||
| + | =Notes= | ||
===Biology=== | ===Biology=== | ||
| − | * David | + | * David Liu has agreed to give us [http://www.nature.com/nature/journal/vaop/ncurrent/full/nature09929.html MP-QUR], pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid. |
* The Elowitz Repressilator can apparently be found on [http://www.addgene.org/pgvec1?f=c&identifier=26489&attag=b&cmd=findpl Add-Gene], will need to either find or figure out a good testing rig to put it in. | * The Elowitz Repressilator can apparently be found on [http://www.addgene.org/pgvec1?f=c&identifier=26489&attag=b&cmd=findpl Add-Gene], will need to either find or figure out a good testing rig to put it in. | ||
* Cory has [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13521 I13521] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522], although both are in pSB1A3, which might prove problematic if the [[Spring_11:Directed_Evolution/KAF95|KAF95]] ''fliC'' is also on a pBR322 derived plasmid. Current backup is [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 J04450] which can be found in pSB1C3 and would allow for a co-transformant. | * Cory has [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13521 I13521] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522], although both are in pSB1A3, which might prove problematic if the [[Spring_11:Directed_Evolution/KAF95|KAF95]] ''fliC'' is also on a pBR322 derived plasmid. Current backup is [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 J04450] which can be found in pSB1C3 and would allow for a co-transformant. | ||
Revision as of 20:57, 18 April 2011
Weekly Progress
Weekend/Monday
- Finalized parts list.
- Continued coding image analysis / segmentation code.
- Fabricated Microfluidics using resources from Kamm lab and Voldemann lab.
- Moved microscope setup to larger breadboard.
Monday Evening
- Will start a culture of I13521 from freezer stock. ~12 hours of grow time should be sufficient. Also, potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the fliC273 gene.
Notes
Biology
- David Liu has agreed to give us MP-QUR, pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid.
- The Elowitz Repressilator can apparently be found on Add-Gene, will need to either find or figure out a good testing rig to put it in.
- Cory has I13521 and I13522, although both are in pSB1A3, which might prove problematic if the KAF95 fliC is also on a pBR322 derived plasmid. Current backup is J04450 which can be found in pSB1C3 and would allow for a co-transformant.
