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• 20.109(F08): Yeast transformation
  ... if...?"</i>. And oddly, spending some time being negative before you jump in is likely to improve your chances of a successful outcome since some of the ...If the controls in an experiment haven't worked there is very little point in considering the data you have collected. Even experienced researchers often
  19 KB (3,254 words) - 19:05, 28 July 2015
• 20.109(F08): Phage nanowires
  ...way to find out from the dinosaurs themselves how the iridium came to rest in this clay layer. ...osive, unmachinable, dinosaur killing metal thats relevant for our work in this module. Iridium, when oxidized to IrO2, has a d-orbital conductance ba
  13 KB (2,275 words) - 19:05, 28 July 2015
• 20.109(F08): Western Analysis
  ...olerated by the phage, specifically, if the modified protein is detectable in bacteria that are infected by your manipulated phage and if the phage life ...sequently, proteins are coated with a detergent ("SDS"---same thing that's in many shampoos) to allow them to be drawn through the electrophoresis matrix
  11 KB (1,723 words) - 19:05, 28 July 2015
• 20.109(F08): Transmission electron microscopy
  ...ral size (250X magnification). Compound microscopes, like the ones we have in lab, use a second lens to magnify the image from the first and can increase ...” then strikes a fluorescent screen, giving rise to an image that varies in darkness according to the sample's density. For samples that are amenable t
  7 KB (1,172 words) - 19:05, 28 July 2015
• 20.109(F08): ECD assembly
  ...ntil the stock is replenished to see all the aspects of this “revolution in book reading.” The promotional material for this handheld book-sized pro ...module), you note how their workings rely on excitation of a material held in tiny cells between panels of glass. The excited materials emit light of par
  13 KB (2,320 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes
  ...ogy has given us the ability to read the 4 letter genetic program of cells in their entirety. Molecular techniques like the ones you've learned so far th Instead of rewriting the DNA in response to every change in its surroundings, a cell will vary the output of its genes, varying their a
  25 KB (4,068 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 7 Lipofection
  ...ld be transcribed by RNA polymerase. The polyadenylation sequence assists in the export and stability of the mRNA so that it gets translated by the ribo ...s introduced into a cell). For stable transfection, the DNA is introduced in such a way that it is maintained indefinitely. Today you will be transient
  10 KB (1,701 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 3 Day 2 Phage nanowires
  ...way to find out from the dinosaurs themselves how the iridium came to rest in this clay layer. ...osive, unmachinable, dinosaur killing metal thats relevant for our work in this module. Iridium, when oxidized to IrO2, has a d-orbital conductance ba
  13 KB (2,079 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 2 Day 1 Protein engineering with PCR
  ...omatin remodeling is required for appropriate gene expression which is, in turn, required for healthy cell behaviors. <br> ... first few days of this module, we'll add a protein tag to some SAGA genes in yeast or some sequences that are regulated by SAGA. If we wanted to genetic
  30 KB (4,904 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 2 Day 2 Yeast transformation
  ... if...?"</i>. And oddly, spending some time being negative before you jump in is likely to improve your chances of a successful outcome since some of the ...If the controls in an experiment haven't worked there is very little point in considering the data you have collected. Even experienced researchers often
  18 KB (3,152 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 2 Day 5 Probe western, isolate RNA
  ...ex. Assuming the protein we're interested in is expressed and intact, then in general, the quality of Western results depends on the quality of the antib ...recognize multiple epitopes. We will be using a polyclonal antibody raised in rabbits against the Protein A from <i>Staphylococcus aureus</i>, but for th
  20 KB (3,266 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 3 Day 6 ECD assembly
  ...mazon's Kindle]][[Image:Epaper.png|thumb|right| Epaper microcapsules]]Just in time for the 2007 holiday shopping season, Amazon.com came out with a wirel ...module), you note how their workings rely on excitation of a material held in tiny cells between panels of glass. The excited materials emit light of par
  12 KB (2,111 words) - 19:06, 28 July 2015
• 20.109(F08):DNA engineering powerpoint pitch guidelines
  *This assignment is due by 11:00 a.m. on the day you have lab. Please turn in your "P3" electronically by emailing it to bevin, nlerner, myapoe, nkuldell *This assignment is submitted as a powerpoint file but you will _not_ have the opportunity t
  2 KB (319 words) - 19:06, 28 July 2015
• 20.109(S09): Luciferase assays and RNA prep (Day6)
  ...sed on what was observable. And amazingly this completely wrong theory was in place for nearly 2000 years. Rudolph Virchow didnt state that where a Coupled with data collection is instrumentation and technology. Advances in technology often lead to scientific progress. (e.g., microscopes that heral
  14 KB (2,345 words) - 19:34, 28 July 2015
• 20.109(F09): Mod 2 Day 3 Tools for system engineering
  ...increase the phosphatase activity of the sensor when the cells are growing in the light. The first class of mutants (we'll call them K+) should make the ...Cph1 to EnvZ, called Cph8, changes the numbering of the residues, as shown in the figure below. It's hoped, however, that the local environment of the re
  24 KB (3,897 words) - 19:39, 28 July 2015
• 20.109(F09): Mod 3 Day 2 Phage nanowires
  [[Image:CoinBattery.jpg|thumb|Coin-type battery]]The materials prepared in this lab have activity as battery electrodes. The redox properties of the ...into a coin type battery and tested. Groups will vary the amount of silver in the gold phage nanowires to see how these variations affect the charge/disc
  7 KB (1,146 words) - 19:39, 28 July 2015
• 20.109(S09):Prepare expression system (Day4)
  ... your oh-so devoted teaching staff picked two colonies per mutant to grow in liquid culture. The XL1-Blue cell line, although it now carries the inverse ...ide). To further reduce leaky expression of the protein of interest (in our case, inverse pericam), the pLysS version of BL21(DE3) contains T7 lyso
  21 KB (3,468 words) - 19:33, 28 July 2015
• 20.109(S09): siRNA design (Day1)
  ...on proteins, and to implement protein modifications, you manipulated DNA. In this experimental module, RNA gets the spotlight. While DNA almost exclusiv ...term used to describe how much of a gene product is synthesized by a cell. In liver cells, expression of genes for liver-specific proteins is high and th
  22 KB (3,613 words) - 19:33, 28 July 2015
• 20.109(F09): Mod 1 Day 6 Restriction map and tissue culture
  ...ause people were doing exactly that, extracting tissue and letting it live in a dish for a short time. Today, most tissue culture experiments are done us ...an divide indefinitely. (Some inherent cell-to-cell variation still exists in such cells.)
  11 KB (1,830 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 7 Lipofection
  ...ld be transcribed by RNA polymerase. The polyadenylation sequence assists in the export and stability of the mRNA so that it gets translated by the ribo ...s introduced into a cell). For stable transfection, the DNA is introduced in such a way that it is maintained indefinitely. Today you will be transient
  10 KB (1,594 words) - 19:38, 28 July 2015