User contributions
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- 16:37, 6 November 2018 (diff | hist) . . (+1) . . 20.109(F18):Measure fermentation products (Day8) (→Part 2: Quantify fermentation product yield)
- 16:36, 6 November 2018 (diff | hist) . . (0) . . File:M2ColorimetricAssay Template.xlsx (Josephinebagnall uploaded a new version of "File:M2ColorimetricAssay Template.xlsx")
- 16:34, 6 November 2018 (diff | hist) . . (-2) . . 20.109(F18):Class data (→Fermentation product and gene targeted:)
- 16:34, 6 November 2018 (diff | hist) . . (+190) . . 20.109(F18):Class data (→Fermentation product and gene targeted:)
- 16:33, 6 November 2018 (diff | hist) . . (+293) . . 20.109(F18):Class data (→Fermentation product and gene targeted:)
- 16:30, 6 November 2018 (diff | hist) . . (+68) . . 20.109(F18):Measure fermentation products (Day8) (→Part 2: Quantify fermentation product yield)
- 16:29, 6 November 2018 (diff | hist) . . (0) . . N File:M2ColorimetricAssay Template.xlsx
- 16:28, 6 November 2018 (diff | hist) . . (+53) . . 20.109(F18):Measure fermentation products (Day8) (→Protocols)
- 16:26, 6 November 2018 (diff | hist) . . (+119) . . 20.109(F18):Measure fermentation products (Day8) (→Protocols)
- 20:48, 5 November 2018 (diff | hist) . . (0) . . 20.109(F18):Measure fermentation products (Day8) (→Part 2: Quantify fermentation product yield)
- 20:45, 5 November 2018 (diff | hist) . . (0) . . 20.109(F18):Measure fermentation products (Day8) (→Part 2: Quantify fermentation product yield)
- 20:35, 5 November 2018 (diff | hist) . . (-1) . . 20.109(F18):Measure fermentation products (Day8) (→Part 2: Quantify fermentation product yield)
- 20:34, 5 November 2018 (diff | hist) . . (0) . . 20.109(F18):Measure fermentation products (Day8) (→Part 2: Quantify fermentation product yield)
- 20:01, 1 November 2018 (diff | hist) . . (0) . . N File:Fa18 M2D7 JBmarked.pdf (current)
- 19:59, 1 November 2018 (diff | hist) . . (+48) . . 20.109(F18): Fall 2018 schedule
- 16:46, 1 November 2018 (diff | hist) . . (-21) . . 20.109(F18):Induce CRISPRi system (Day7) (→Protocols) (current)
- 16:44, 1 November 2018 (diff | hist) . . (+2) . . 20.109(F18):Class data (→Module 2)
- 15:51, 1 November 2018 (diff | hist) . . (0) . . N File:Fa18 TR Purple M2 seq.zip (current)
- 15:51, 1 November 2018 (diff | hist) . . (0) . . N File:Fa18 TR Pink M2 seq.zip (current)
- 15:50, 1 November 2018 (diff | hist) . . (0) . . N File:Fa18 TR Green M2 seq.zip (current)
- 15:50, 1 November 2018 (diff | hist) . . (0) . . N File:Fa18 TR Orange M2 seq.zip (current)
- 15:50, 1 November 2018 (diff | hist) . . (0) . . N File:Fa18 TR Red M2 seq.zip (current)
- 12:18, 1 November 2018 (diff | hist) . . (+598) . . 20.109(F18):Class data (→Module 2)
- 01:53, 1 November 2018 (diff | hist) . . (+83) . . 20.109(F18):Homework (→Due M2D8)
- 21:08, 30 October 2018 (diff | hist) . . (+276) . . 20.109(F18):Class data (→Fermentation product and gene targeted:)
- 19:58, 29 October 2018 (diff | hist) . . (+13) . . 20.109(F18):Induce CRISPRi system (Day7) (→Part 2: Examine pgRNA sequencing results)
- 18:26, 25 October 2018 (diff | hist) . . (+1) . . 20.109(F18):Confirm gRNA sequence (Day5) (current)
- 18:17, 25 October 2018 (diff | hist) . . (0) . . N File:Fa18 M2D5 JBmarked.pdf (current)
- 18:15, 25 October 2018 (diff | hist) . . (+48) . . 20.109(F18): Fall 2018 schedule
- 16:18, 25 October 2018 (diff | hist) . . (+49) . . 20.109(F18):Homework (→Due M2D5)
- 16:08, 25 October 2018 (diff | hist) . . (+133) . . 20.109(F18):Homework (→Due M2D7)
- 15:19, 25 October 2018 (diff | hist) . . (+54) . . 20.109(F18):Confirm gRNA sequence (Day5)
- 14:40, 25 October 2018 (diff | hist) . . (+115) . . 20.109(F18):Confirm gRNA sequence (Day5) (→Part 1: Mini-prep pgRNA_target clones)
- 14:27, 24 October 2018 (diff | hist) . . (+39) . . 20.109(F18):Confirm gRNA sequence (Day5) (→Part 3: Prepare pgRNA clones for sequencing analysis)
- 14:23, 24 October 2018 (diff | hist) . . (-4) . . 20.109(F18):Confirm gRNA sequence (Day5) (→Part 3: Prepare pgRNA clones for sequencing analysis)
- 19:14, 18 October 2018 (diff | hist) . . (0) . . N File:Fa18 M2D3 JBmarked.pdf (current)
- 19:14, 18 October 2018 (diff | hist) . . (0) . . N File:M2D2 Fa18 LMmarked.pdf (current)
- 19:13, 18 October 2018 (diff | hist) . . (0) . . 20.109(F18): Fall 2018 schedule
- 18:55, 18 October 2018 (diff | hist) . . (+97) . . 20.109(F18): Fall 2018 schedule
- 19:05, 16 October 2018 (diff | hist) . . (-1) . . 20.109(F18):Design gRNA for CRISPRi (Day2) (→Part 2: Select E. coli fermentation pathway protein to target)
- 19:04, 16 October 2018 (diff | hist) . . (-31) . . 20.109(F18):Design gRNA for CRISPRi (Day2) (→Part 2: Select E. coli fermentation pathway protein to target)
- 18:21, 16 October 2018 (diff | hist) . . (0) . . N File:Fa18 M2D2 JBmarked.pdf (current)
- 18:21, 16 October 2018 (diff | hist) . . (-4) . . 20.109(F18): Fall 2018 schedule
- 18:20, 16 October 2018 (diff | hist) . . (+48) . . 20.109(F18): Fall 2018 schedule
- 18:09, 16 October 2018 (diff | hist) . . (0) . . 20.109(F18):Design gRNA for CRISPRi (Day2)
- 20:11, 15 October 2018 (diff | hist) . . (-42) . . 20.109(F18):Generate gRNA plasmid (Day3) (→Part 3: Complete gRNA insertion and amplification reaction) (current)
- 19:53, 15 October 2018 (diff | hist) . . (-22) . . 20.109(F18):Generate gRNA plasmid (Day3) (→Part 2: gRNA oligo preparation)
- 19:50, 15 October 2018 (diff | hist) . . (-26) . . 20.109(F18):Design gRNA for CRISPRi (Day2) (→Part 3: Design gRNA for CRISPRi system)
- 18:46, 15 October 2018 (diff | hist) . . (+49) . . 20.109(F18):Homework (→Wrapping up M2)
- 18:44, 15 October 2018 (diff | hist) . . (-20) . . 20.109(F18):Homework (→Wrapping up M3)
- 18:43, 15 October 2018 (diff | hist) . . (0) . . 20.109(F18):Homework (→Wrapping up M2)
- 18:43, 15 October 2018 (diff | hist) . . (0) . . 20.109(F18):Homework (→Wrapping up M1)
- 18:42, 15 October 2018 (diff | hist) . . (-3) . . 20.109(F18):Homework (→Wrapping up M1)
- 18:42, 15 October 2018 (diff | hist) . . (+6) . . 20.109(F18):Homework (→Wrapping up M1)
- 17:32, 15 October 2018 (diff | hist) . . (+12) . . 20.109(F18):Class data (→Fermentation product and gene targeted:)
- 17:31, 15 October 2018 (diff | hist) . . (+536) . . 20.109(F18):Class data
- 17:28, 15 October 2018 (diff | hist) . . (-32) . . 20.109(F18):Design gRNA for CRISPRi (Day2) (→Part 3: Design gRNA for CRISPRi system)
- 20:21, 12 October 2018 (diff | hist) . . (+5) . . 20.109(F18):Journal club presentation (Day4 and 6) (→Presentation day reservation)
- 20:17, 11 October 2018 (diff | hist) . . (0) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 3: Ligation of dCas9 insert and expression vector)
- 19:08, 11 October 2018 (diff | hist) . . (+8) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 4: Confirmation digest)
- 19:07, 11 October 2018 (diff | hist) . . (-5) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 4: Confirmation digest)
- 17:51, 11 October 2018 (diff | hist) . . (-4) . . 20.109(F18): Fall 2018 schedule
- 17:50, 11 October 2018 (diff | hist) . . (0) . . N File:Fa18 M2D1 JBmarked.pdf (current)
- 17:49, 11 October 2018 (diff | hist) . . (+48) . . 20.109(F18): Fall 2018 schedule
- 15:54, 11 October 2018 (diff | hist) . . (+13) . . 20.109(F18):Homework (→Due M2D2)
- 15:20, 11 October 2018 (diff | hist) . . (0) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 3: Ligation of dCas9 insert and expression vector)
- 18:41, 10 October 2018 (diff | hist) . . (-5) . . 20.109(F18):Complete in silico cloning (Day1) (→Reagents)
- 16:48, 10 October 2018 (diff | hist) . . (+12) . . 20.109(F18):Journal club presentation (Day4 and 6) (→Presentation day reservation)
- 16:47, 10 October 2018 (diff | hist) . . (-45) . . 20.109(F18):Journal club presentation (Day4 and 6) (→Presentation day reservation)
- 16:42, 10 October 2018 (diff | hist) . . (-21) . . 20.109(F18):Homework (→Due M2D2)
- 16:09, 10 October 2018 (diff | hist) . . (+57) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 4: Confirmation digest)
- 16:00, 10 October 2018 (diff | hist) . . (-2) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 4: Confirmation digest)
- 16:00, 10 October 2018 (diff | hist) . . (+1) . . 20.109(F18):Complete in silico cloning (Day1)
- 15:58, 10 October 2018 (diff | hist) . . (-38) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 4: Confirmation digest)
- 15:49, 10 October 2018 (diff | hist) . . (+141) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 3: Ligation of dCas9 insert and expression vector)
- 15:28, 10 October 2018 (diff | hist) . . (+145) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 15:26, 10 October 2018 (diff | hist) . . (-48) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 15:23, 10 October 2018 (diff | hist) . . (-76) . . 20.109(F18):Complete in silico cloning (Day1) (→Protocols)
- 15:09, 10 October 2018 (diff | hist) . . (+62) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 15:00, 10 October 2018 (diff | hist) . . (+1) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 15:00, 10 October 2018 (diff | hist) . . (+26) . . 20.109(F18):Complete in silico cloning (Day1) (→Part 1: PCR amplification and restriction enzyme digest of dCas9 insert)
- 00:04, 10 October 2018 (diff | hist) . . (+4) . . 20.109(F18): Fall 2018 schedule
- 21:27, 4 October 2018 (diff | hist) . . (0) . . N File:181004TRgreenH2AX analyzed.xlsx
- 20:52, 4 October 2018 (diff | hist) . . (+35) . . 20.109(F18):Class data (→gamma-H2AX Data)
- 20:51, 4 October 2018 (diff | hist) . . (+52) . . 20.109(F18):Class data (→gamma-H2AX Data)
- 19:34, 4 October 2018 (diff | hist) . . (0) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Test gamma-H2AX quantification on one representative image) (current)
- 18:36, 4 October 2018 (diff | hist) . . (0) . . N File:Fa18 M1D7 JBmarked.pdf (current)
- 18:33, 4 October 2018 (diff | hist) . . (+48) . . 20.109(F18): Fall 2018 schedule
- 18:45, 3 October 2018 (diff | hist) . . (-1) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Part 5: Draft first data slide for Data summary)
- 18:44, 3 October 2018 (diff | hist) . . (0) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Part 5: Draft first data slide for Data summary)
- 18:40, 3 October 2018 (diff | hist) . . (+1,014) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)
- 17:22, 3 October 2018 (diff | hist) . . (-35) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Part 4: Overlay H2AX channels to make a single color image)
- 17:21, 3 October 2018 (diff | hist) . . (+12) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Part 4: Overlay H2AX channels to make a single color image)
- 16:57, 3 October 2018 (diff | hist) . . (+313) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Part 4: Overlay H2AX channels to make a single color image)
- 16:49, 3 October 2018 (diff | hist) . . (+457) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Part 4: Overlay H2AX channels to make a single color image)
- 16:45, 3 October 2018 (diff | hist) . . (+4) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Quantify gamma-H2AX signal in all images)
- 16:45, 3 October 2018 (diff | hist) . . (+80) . . 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7) (→Quantify gamma-H2AX signal in all images)
- 16:43, 3 October 2018 (diff | hist) . . (+2) . . 20.109(F18):Class data (→Module 1)
- 16:42, 3 October 2018 (diff | hist) . . (+60) . . 20.109(F18):Class data (→gamma-H2AX Data)
- 16:41, 3 October 2018 (diff | hist) . . (+1,224) . . 20.109(F18):Class data (→Module 1)
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