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20.109(S07):Guidelines for working in the tissue culture facility20.109(S07):Guidelines for writing a lab report20.109(S07):Lab tour
20.109(S07):OpenWetWare20.109(S07):Start-up genome engineering20.109(S07): Agarose gel electrophoresis
20.109(S07): Bio-material engineering/Optimizing panning20.109(S07): Bio-material engineering/PCR of gold binding candidates20.109(S07): Bio-material engineering/Rescreening gold binders
20.109(S07): Bio-material engineering/Sequence analysis20.109(S07): Building phage nanowires20.109(S07): Calcium signaling in vivo
20.109(S07): Colony PCR20.109(S07): Expression engineering report20.109(S07): Genome engineering essay
20.109(S07): Growth of phage materials20.109(S07): Lipofection20.109(S07): Measuring calcium in vitro
20.109(S07): Measuring calcium in vivo20.109(S07): Microarray data analysis20.109(S07): Start-up expression engineering
20.109(S07): Start-up signal measurement20.109(S07): Student presentations, module 220.109(S07): Testing redesigned genomes
20.109(S07): Western analysis20.109(S07): Yeast transformation20.109(S07): cDNA synthesis and microarray
20.109(S08)20.109(S08):Characterize protein expression (Day5)20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)
20.109(S08):DNA engineering/Clean and cut DNA (Day 2)20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
20.109(S08):DNA engineering/Examine candidate clones (Day 5)20.109(S08):DNA engineering/FACS analysis (Day 8)20.109(S08):DNA engineering/Lab report
20.109(S08):DNA engineering/Lipofection (Day 7)20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6)20.109(S08):Data analysis (Day7)
20.109(S08):General lab policy20.109(S08):Guidelines for editing OpenWetWare20.109(S08):Guidelines for maintaining your lab notebook
20.109(S08):Guidelines for oral presentations20.109(S08):Guidelines for working in the tissue culture facility20.109(S08):Guidelines for writing a lab report
20.109(S08):Induce protein expression (Day4)20.109(S08):Initiate cell culture (Day2)20.109(S08):Lab basics
20.109(S08):Lab tour20.109(S08):Module 120.109(S08):Module 2
20.109(S08):Module 320.109(S08):OWW basics20.109(S08):OpenWetWare
20.109(S08):People20.109(S08):Prepare expression system (Day3)20.109(S08):Preparing cells for analysis (Day4)
20.109(S08):Protein-level analysis (Day6)20.109(S08):Site-directed mutagenesis (Day2)20.109(S08):Start-up biomaterials engineering (Day1)
20.109(S08):Start-up protein engineering (Day1)20.109(S08):Student presentations (M2D8)20.109(S08):Testing cell viability (Day3)
20.109(S08):Transcript-level analysis (Day5)20.109(S08):Wrap-up analysis (Day7)20.109(S08): Assignments
20.109(S08): Fall 2007 schedule20.109(S08): Spring 2008 schedule20.109(S08): TA notes for module 1
20.109(S08): TA notes for module 220.109(S09)20.109(S09):Assay protein behavior (Day7)
20.109(S09):Bacterial amplification of DNA (Day3)20.109(S09):Characterize protein expression (Day6)20.109(S09):Data analysis (Day8)
20.109(S09):General lab policy20.109(S09):Guidelines for maintaining your lab notebook20.109(S09):Guidelines for oral presentations
20.109(S09):Guidelines for writing up your research20.109(S09):Induce protein and evaluate DNA (Day5)20.109(S09):Lab basics
20.109(S09):Module 120.109(S09):Module 220.109(S09):Module 3
20.109(S09):OpenWetWare20.109(S09):People20.109(S09):Prepare expression system (Day4)
20.109(S09):Protein-level analysis (Day6)20.109(S09):Site-directed mutagenesis (Day2)20.109(S09):Start-up biomaterials engineering (Day1)
20.109(S09):Start-up protein engineering (Day1)20.109(S09):Wrap-up analysis (Day7)20.109(S09): Journal article discussion (Day2)
20.109(S09): Luciferase assays and RNA prep (Day6)20.109(S09): Microarray data analysis (Day8)20.109(S09): Module 2 oral presentations
20.109(S09): Spring 2009 schedule20.109(S09): Transfection (Day5)20.109(S09): cDNA synthesis and microarray (Day7)
20.109(S09): siRNA design (Day1)20.109(S10)20.109(S10):Amplify aptamer-encoding DNA (Day1)
20.109(S10):Aptamer binding assay (Day7)20.109(S10):Assay protein behavior (Day7)20.109(S10):Bacterial amplification of DNA (Day3)
20.109(S10):Characterize protein expression (Day6)20.109(S10):Data analysis (Day8)20.109(S10):General lab policy
20.109(S10):Guidelines for editing OpenWetWare20.109(S10):Guidelines for maintaining your lab notebook20.109(S10):Guidelines for oral presentations
20.109(S10):Guidelines for working in the tissue culture facility20.109(S10):Guidelines for writing up your research20.109(S10):Induce protein and evaluate DNA (Day5)
20.109(S10):Initiate cell culture (Day2)20.109(S10):Journal club (Day8)20.109(S10):Lab basics
20.109(S10):Lab tour20.109(S10):Module 120.109(S10):Module 2
20.109(S10):Module 320.109(S10):OWW basics20.109(S10):OpenWetWare
20.109(S10):People20.109(S10):Post-selection IVT and journal club (Day6)20.109(S10):Prepare RNA by IVT (Day3)
20.109(S10):Prepare expression system (Day4)20.109(S10):Preparing cells for analysis (Day4)20.109(S10):Protein-level analysis (Day6)
20.109(S10):Purify RNA and run affinity column (Day4)20.109(S10):Purify aptamer-encoding DNA (Day2)20.109(S10):RNA to DNA by RT-PCR (Day5)
20.109(S10):Site-directed mutagenesis (Day2)20.109(S10):Start-up biomaterials engineering (Day1)20.109(S10):Start-up protein engineering (Day1)
20.109(S10):Statement on Collaboration and Integrity20.109(S10):Student presentations (Day8)20.109(S10):Testing cell viability (Day3)
20.109(S10):Transcript-level analysis (Day5)20.109(S10):Wrap-up analysis (Day7)20.109(S10): Assignments
20.109(S10): Spring 2010 schedule20.109(S11)20.109(S11):Amplify aptamer-encoding DNA (Day1)
20.109(S11):Aptamer binding assay (Day7)20.109(S11):Complete DNA design (Day2)20.109(S11):General lab policy
20.109(S11):Guidelines for editing OpenWetWare20.109(S11):Guidelines for maintaining your lab notebook20.109(S11):Guidelines for oral presentations
20.109(S11):Guidelines for working in the tissue culture facility20.109(S11):Guidelines for writing up your research20.109(S11):Journal club (Day8)
20.109(S11):Lab basics20.109(S11):Lab tour20.109(S11):Ligate DNA and transform bacteria (Day4)
20.109(S11):Module 120.109(S11):Module 220.109(S11):Module 3
20.109(S11):Module 3 oral presentations20.109(S11):OWW basics20.109(S11):OpenWetWare
20.109(S11):People20.109(S11):Post-selection IVT and journal club (Day6)20.109(S11):Prepare DNA for cloning (Day3)
20.109(S11):Prepare RNA by IVT (Day3)20.109(S11):Purify RNA and run affinity column (Day4)20.109(S11):Purify aptamer-encoding DNA (Day2)
20.109(S11):RNA to DNA by RT-PCR (Day5)20.109(S11):Start-up biomaterials engineering (Day1)20.109(S11):Statement on Collaboration and Integrity
20.109(S11):Student presentations (Day8)20.109(S11): Assignments20.109(S11): RNA computational analysis
20.109(S11): RNA engineering report20.109(S11): Spring 2010 schedule20.109(S11): TA notes for module 1
20.109(S11): TA notes for module 220.109(S11): TA notes for module 320.109(S12)
20.109(S12):Amplify aptamer-encoding DNA (Day1)20.109(S12):Aptamer binding assay (Day7)20.109(S12):Assess protein function (Day7)
20.109(S12):Bacterial amplification of DNA (Day3)20.109(S12):Characterize protein expression (Day6)20.109(S12):FNT summary page
20.109(S12):General lab policy20.109(S12):Guidelines for maintaining your lab notebook20.109(S12):Guidelines for oral presentations
20.109(S12):Guidelines for working in the tissue culture facility20.109(S12):Guidelines for writing up your research20.109(S12):Induce protein and evaluate DNA (Day5)
20.109(S12):Initiate cell culture (Day2)20.109(S12):Initiating transcript and protein assays (Day5)20.109(S12):Journal club (Day8)
20.109(S12):Lab tour20.109(S12):Module 120.109(S12):Module 2
20.109(S12):Module 320.109(S12):Module 3 oral presentations20.109(S12):OWW in 20.109
20.109(S12):People20.109(S12):Post-selection IVT and journal club (Day6)20.109(S12):Prepare RNA by IVT (Day3)
20.109(S12):Prepare expression system (Day4)20.109(S12):Preparing cells for analysis (Day4)20.109(S12):Protein-level and wrap-up analysis (Day7)
20.109(S12):Purify RNA and run affinity column (Day4)20.109(S12):Purify aptamer-encoding DNA (Day2)20.109(S12):RNA to DNA by RT-PCR (Day5)
20.109(S12):Reflection assignments summary page20.109(S12):Site-directed mutagenesis (Day2)20.109(S12):Start-up biomaterials engineering (Day1)
20.109(S12):Start-up protein engineering (Day1)20.109(S12):Statement on collaboration and integrity20.109(S12):Student presentations (Day8)
20.109(S12):Testing cell viability (Day3)20.109(S12):Transcript-level analysis (Day6)20.109(S12): Assignments
20.109(S12): Protein engineering report20.109(S12): RNA computational analysis20.109(S12): RNA engineering report
20.109(S12): Spring 2012 schedule20.109(S12): TA notes for module 120.109(S13)
20.109(S13):Assess protein function (Day8)20.109(S13):Bacterial amplification of DNA (Day3)20.109(S13):Characterize protein expression (Day7)
20.109(S13):Context-setting and primer design (Day1)20.109(S13):DNA cloning (Day4)20.109(S13):DNA extraction (Day2)
20.109(S13):DNA sequencing and primer analysis (Day5)20.109(S13):Design experiment (Day1)20.109(S13):Design mutant (Day1)
20.109(S13):General lab policy20.109(S13):Guidelines for maintaining your lab notebook20.109(S13):Guidelines for oral presentations
20.109(S13):Guidelines for working in the tissue culture facility20.109(S13):Guidelines for writing up your research20.109(S13):Induce protein and evaluate DNA (Day5)
20.109(S13):Initiate cell culture (Day2)20.109(S13):Initiating transcript and protein assays (Day5)20.109(S13):Journal club II (Day8)
20.109(S13):Journal club I (Day6)20.109(S13):Lab tour20.109(S13):Module 1
20.109(S13):Module 220.109(S13):Module 320.109(S13):Module 3 oral presentations
20.109(S13):OWW in 20.10920.109(S13):Optional gel purification20.109(S13):PCR and paper discussion (Day3)
20.109(S13):People20.109(S13):Phylogenetic analysis (Day7)20.109(S13):Prepare expression system (Day4)
20.109(S13):Preparing cells for analysis (Day4)20.109(S13):Protein-level and wrap-up analysis (Day7)20.109(S13):Purify protein (Day6)
20.109(S13):Reflection assignments summary page20.109(S13):Site-directed mutagenesis (Day2)20.109(S13):Statement on collaboration and integrity
20.109(S13):Student presentations (Day8)20.109(S13):Testing cell viability (Day3)20.109(S13):Transcript-level analysis (Day6)
20.109(S13): Assignments20.109(S13): Cell-biomaterial engineering report20.109(S13): DNA engineering report
20.109(S13): Primer design summary20.109(S13): Protein engineering report20.109(S13): Spring 2013 schedule
20.109(S13): TA notes for module 120.109(S14)20.109(S14):16S PCR and paper discussion (Day3)
20.109(S14):Begin Western protein analysis (Day2)20.109(S14):Cell preparation for DNA repair assays (Day5)20.109(S14):Complete Western and prepare damaged DNA (Day4)
20.109(S14):DNA cloning (Day4)20.109(S14):DNA repair assays(Day6)20.109(S14):DNA sequencing (Day5)
20.109(S14):Data analysis (Day7)20.109(S14):Design experiment (Day1)20.109(S14):Diagnostic primer design (Day2)
20.109(S14):General lab policy20.109(S14):Guidelines for maintaining your lab notebook20.109(S14):Guidelines for oral presentations
20.109(S14):Guidelines for working in the tissue culture facility20.109(S14):Guidelines for writing up your research20.109(S14):Initiate cell culture (Day2)
20.109(S14):Initiating transcript and protein assays (Day5)20.109(S14):Introduction to cell strains and plating (Day1)20.109(S14):Journal club II (Day8)
20.109(S14):Journal club I (Day6)20.109(S14):Lab tour20.109(S14):Microbial DNA extraction (Day1)
20.109(S14):Microbiome summary20.109(S14):Module 120.109(S14):Module 2
20.109(S14):Module 320.109(S14):Module 3 oral presentations20.109(S14):NHEJ system research article
20.109(S14):OWW in 20.10920.109(S14):People20.109(S14):Phylogenetic and primer analyses (Day7)
20.109(S14):Preparing cells for analysis (Day4)20.109(S14):Primer design memo20.109(S14):Protein-level and wrap-up analysis (Day7)
20.109(S14):Reflection assignments summary page20.109(S14):Statement on collaboration and integrity20.109(S14):Student presentations (Day8)
20.109(S14):Testing cell viability (Day3)20.109(S14):Transcript-level analysis (Day6)20.109(S14): Assignments
20.109(S14): Cell engineering mini-report20.109(S14): Choose system conditions and paper discussion (Day3)20.109(S14): Spring 2013 schedule
20.109(S14): Spring 2014 schedule20.109(S14): TA notes for module 120.109(S14): TA notes for module 2
20.109(S14): TA notes for module 320.109(S15)20.109(S15):16S PCR and paper discussion (Day3)
20.109(S15):20.109 Class Blog summary page20.109(S15):AIV detection assay and analysis (Day7)20.109(S15):Begin Western protein analysis and choose system conditions (Day2)
20.109(S15):Biotemplating on phage nanowires (Day 2)20.109(S15):Cell preparation for DNA repair assays (Day4)20.109(S15):DNA cloning (Day4)
20.109(S15):DNA repair assays (Day5)20.109(S15):DNA sequencing (Day5)20.109(S15):Data analysis (Day7)
20.109(S15):Data analysis and paper discussion (Day6)20.109(S15):Diagnostic primer analysis (Day8)20.109(S15):Diagnostic primer design (Day2)
20.109(S15):Flow cytometry and paper discussion (Day6)20.109(S15):General lab policy20.109(S15):Growth of phage nanowires (Day 1)
20.109(S15):Guidelines for maintaining your lab notebook20.109(S15):Guidelines for oral presentations20.109(S15):Guidelines for working in the tissue culture facility
20.109(S15):Guidelines for writing up your research20.109(S15):Introduction to cell strains and plating (Day1)20.109(S15):Journal club II (Day9)
20.109(S15):Journal club I (Day6)20.109(S15):Lab basics20.109(S15):Lab tour
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