Difference between revisions of "Resource list: DNA melting and PCR"
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| − | ==Use of fluorescent | + | ==Use of fluorescent reportes to measure DNA melting curves== |
* Ririe, et al. [http://www.sciencedirect.com/science/article/B6W9V-45MH2GK-G7/2/d2bf78d53a161a9793a248ec388d4659 Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction]. ''Analytical Biochemistry'' 245, 154–160 (1997) | * Ririe, et al. [http://www.sciencedirect.com/science/article/B6W9V-45MH2GK-G7/2/d2bf78d53a161a9793a248ec388d4659 Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction]. ''Analytical Biochemistry'' 245, 154–160 (1997) | ||
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==Applications of DNA melting curves== | ==Applications of DNA melting curves== | ||
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* Worm, et. al. [http://www.gene-quantification.org/worm-hrm-2001.pdf In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis] Clinical Chemistry 47:7 1183–1189 (2001) | * Worm, et. al. [http://www.gene-quantification.org/worm-hrm-2001.pdf In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis] Clinical Chemistry 47:7 1183–1189 (2001) | ||
*[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) (Also see: Ahsen, et. al. [http://www.clinchem.org/cgi/content/full/47/7/1331#R1 Limitations of Genotyping Based on Amplicon Melting Temperature.] Clinical Chemistry. 2001;47:1331-1332. | *[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) (Also see: Ahsen, et. al. [http://www.clinchem.org/cgi/content/full/47/7/1331#R1 Limitations of Genotyping Based on Amplicon Melting Temperature.] Clinical Chemistry. 2001;47:1331-1332. | ||
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| + | ==Real-time PCR== | ||
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| + | * Kubista, et. al. [http://www.tataa.com/files/documents/molecularaspectsofmedicine2006.pdf The real-time polymerase chain reaction] ''Molecular Aspects of Medicine'' 27 (2006) 95–125. | ||
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Revision as of 23:46, 27 May 2010
Use of fluorescent reportes to measure DNA melting curves
- Ririe, et al. Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction. Analytical Biochemistry 245, 154–160 (1997)
- Michael T. Bjorndal and D. Kuchnir Fygenson. DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay. Biopolymers, Vol. 65, 40–44 (2002)
Applications of DNA melting curves
- Worm, et. al. In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis Clinical Chemistry 47:7 1183–1189 (2001)
- Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome. Clinical Chemistry. 2000;46:423-425.) (Also see: Ahsen, et. al. Limitations of Genotyping Based on Amplicon Melting Temperature. Clinical Chemistry. 2001;47:1331-1332.
Real-time PCR
- Kubista, et. al. The real-time polymerase chain reaction Molecular Aspects of Medicine 27 (2006) 95–125.
