Difference between revisions of "Resource list: DNA melting and PCR"
From Course Wiki
(→Use of fluorescent dyes to measure DNA melting curves) |
(→Applications of DNA melting curves) |
||
| Line 17: | Line 17: | ||
* Worm, et. al. [http://www.gene-quantification.org/worm-hrm-2001.pdf In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis] Clinical Chemistry 47:7 1183–1189 (2001) | * Worm, et. al. [http://www.gene-quantification.org/worm-hrm-2001.pdf In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis] Clinical Chemistry 47:7 1183–1189 (2001) | ||
| − | *[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) | + | *[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) (Also see: Ahsen, et. al. [http://www.clinchem.org/cgi/content/full/47/7/1331#R1 Limitations of Genotyping Based on Amplicon Melting Temperature.] Clinical Chemistry. 2001;47:1331-1332. |
| − | + | </div> | |
Revision as of 17:01, 27 May 2010
Use of fluorescent dyes to measure DNA melting curves
- Ririe, et al. Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction. Analytical Biochemistry 245, 154–160 (1997)
- Michael T. Bjorndal and D. Kuchnir Fygenson. DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay. Biopolymers, Vol. 65, 40–44 (2002)
Applications of DNA melting curves
- Worm, et. al. In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis Clinical Chemistry 47:7 1183–1189 (2001)
- Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome. Clinical Chemistry. 2000;46:423-425.) (Also see: Ahsen, et. al. Limitations of Genotyping Based on Amplicon Melting Temperature. Clinical Chemistry. 2001;47:1331-1332.
