Difference between revisions of "Resource list: DNA melting and PCR"
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* Michael T. Bjorndal and D. Kuchnir Fygenson. [http://www.physics.ucsb.edu/~deborah/pub/BjorndalBIOPOLYv65p40y02.pdf DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay]. ''Biopolymers'', Vol. 65, 40–44 (2002) | * Michael T. Bjorndal and D. Kuchnir Fygenson. [http://www.physics.ucsb.edu/~deborah/pub/BjorndalBIOPOLYv65p40y02.pdf DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay]. ''Biopolymers'', Vol. 65, 40–44 (2002) | ||
| − | * [http://www.ambion.com/techlib/tn/81/813.html Applied Biosystems discussion of real-time PCR vs. TaqMan assays] | + | * [http://www.ambion.com/techlib/tn/81/813.html Applied Biosystems discussion of real-time PCR with SYBR Green I vs. TaqMan assays] |
| + | ==Applications of DNA melting curves== | ||
| + | *[http://www.clinchem.org/cgi/content/full/46/3/423?ijkey=61c477a414787cde9c8b650bd108c179ec230248&keytype2=tf_ipsecsha Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome.] ''Clinical Chemistry''. 2000;46:423-425.) | ||
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Revision as of 16:34, 27 May 2010
Use of fluorescent dyes to measure DNA melting curves
- Michael T. Bjorndal and D. Kuchnir Fygenson. DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay. Biopolymers, Vol. 65, 40–44 (2002)
Applications of DNA melting curves
- Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome. Clinical Chemistry. 2000;46:423-425.)
