Difference between revisions of "QRT-PCR"
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| − | + | <!-- ''[[QRT-PCR:Get_Started | Click here for the quick-start guide]]'' --> | |
| − | + | [[File:Pcr-setup.jpg|200px|center]] | |
| + | <div class="center" style="width:auto; margin-left:auto; margin-right:auto;"><big>20.345: QRT-PCR</big></div> | ||
| − | + | == Current State == | |
| − | + | [[File:Pcr-check-1.jpg|thumb|left|300px|taken 5/8/11]] | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | === Optics === | |
| − | + | Auto-fluorescence with the fiber optic is still a problem. Although one option is to keep looking for a fiber with sufficiently low auto-fluorescence levels, it may be more worthwhile to redesign the optics setup so that a fiber optic is no longer needed. | |
| − | + | ||
| − | + | If this latter route is taken, top-down reading (i.e., reading the fluorescence through the cap of the PCR tube) will likely be the best way to avoid unwanted noise from the illuminator. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | * | + | === Electronics === |
| − | * | + | |
| − | * | + | Thermistors are from Quality Thermistor Inc. The ones currently in the machine are not all the same, however. |
| + | |||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! Location | ||
| + | ! Part No. | ||
| + | ! R @ 25C | ||
| + | ! R_detection | ||
| + | ! Steinhart coefficients | ||
| + | |- | ||
| + | | Block | ||
| + | | QTMB-14C3 | ||
| + | | 10 kΩ | ||
| + | | 0.985 MΩ | ||
| + | | | ||
| + | * A: 0.001119391230554 | ||
| + | * B: 0.000236073313265 | ||
| + | * C: 0.000000076143987 | ||
| + | |- | ||
| + | | Sample | ||
| + | | QTMB-16C3 | ||
| + | | 15 kΩ | ||
| + | | 0.987 MΩ | ||
| + | | | ||
| + | * A: 0.001035870602276 | ||
| + | * B: 0.000233384648786 | ||
| + | * C: 0.000000083184364 | ||
| + | |- | ||
| + | | Lid | ||
| + | | QTMB-16C3 | ||
| + | | 15 kΩ | ||
| + | | 0.999 MΩ | ||
| + | | | ||
| + | * A: 0.001035870602276 | ||
| + | * B: 0.000233384648786 | ||
| + | * C: 0.000000083184364 | ||
| + | |} | ||
| + | |||
| + | === LabView === | ||
| + | ''Go to [[QRT-PCR:LabView-versions | LabView QRT-PCR version info]] page'' | ||
| + | |||
| + | Most useful versions in the course-share: | ||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! Version No. | ||
| + | ! | ||
| + | |- | ||
| + | | 2.52 | ||
| + | | '''Oscillator Thermistor:''' Will oscillate the TEC at a user-defined frequency. Useful for constructing Bode plots for the thermal circuit of the machine. | ||
| + | |- | ||
| + | | 2.712 | ||
| + | | '''Sampling:''' "Samples" the fluoresence by flashing the excitation LED on/off at a user-defined frequency. It also has the best version (as of May 2011) of the TEC controller. | ||
| + | |} | ||
| + | |||
| + | == Past Iterations == | ||
| + | |||
| + | ''Go to [[Spring 11:QRT-PCR|Spring 2011 Progress Details]].'' | ||
| + | |||
| + | == Resources == | ||
| + | === Research Notes === | ||
| + | * [[QRT-PCR:Heated Lid | Heated Lid]] | ||
| + | |||
| + | === Useful External Links === | ||
| + | * [http://www.heatersplus.com/wire.htm Resistance wire temperature table ] | ||
| + | * [http://www.heatersplus.com/nichrome.htm Calculations with resistance wire ] | ||
| + | * [http://learnlabview.blogspot.com/search/label/0010%20-%20Programming%20LabView%20-%20Arrays Simple Labview Tutorial ] | ||
| + | * [http://pathmicro.med.sc.edu/pcr/realtime-home.htm QRT-PCR Tutorial] | ||
Latest revision as of 17:28, 19 May 2011
20.345: QRT-PCR
Contents
Current State
Optics
Auto-fluorescence with the fiber optic is still a problem. Although one option is to keep looking for a fiber with sufficiently low auto-fluorescence levels, it may be more worthwhile to redesign the optics setup so that a fiber optic is no longer needed.
If this latter route is taken, top-down reading (i.e., reading the fluorescence through the cap of the PCR tube) will likely be the best way to avoid unwanted noise from the illuminator.
Electronics
Thermistors are from Quality Thermistor Inc. The ones currently in the machine are not all the same, however.
| Location | Part No. | R @ 25C | R_detection | Steinhart coefficients |
|---|---|---|---|---|
| Block | QTMB-14C3 | 10 kΩ | 0.985 MΩ |
|
| Sample | QTMB-16C3 | 15 kΩ | 0.987 MΩ |
|
| Lid | QTMB-16C3 | 15 kΩ | 0.999 MΩ |
|
LabView
Go to LabView QRT-PCR version info page
Most useful versions in the course-share:
| Version No. | |
|---|---|
| 2.52 | Oscillator Thermistor: Will oscillate the TEC at a user-defined frequency. Useful for constructing Bode plots for the thermal circuit of the machine. |
| 2.712 | Sampling: "Samples" the fluoresence by flashing the excitation LED on/off at a user-defined frequency. It also has the best version (as of May 2011) of the TEC controller. |
Past Iterations
Go to Spring 2011 Progress Details.
