Optical microscopy lab wiki pages

Optical microscopy lab

Lab manuals

• Optical Microscopy Overview
• Optical Microscopy Part 1: Brightfield Microscopy
• Optical Microscopy Part 2: Fluorescence Microscopy
• Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking
• Optical Microscopy Report Outline

Code examples and simulations

• Converting Gaussian fit to Rayleigh resolution
• MATLAB: Estimating resolution from a PSF slide image
• Matlab: Scalebars
• Calculating MSD and Diffusion Coefficients

Background reading

• Geometrical optics and ray tracing
• Physical optics and resolution
• Optical aberrations
• Aperture and field stops
• Optical detectors, noise, and the limit of detection
• Manta G032 camera measurements