Difference between revisions of "Optical Microscopy: Part 3 Report Outline"
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<li>Data</li> | <li>Data</li> | ||
<ul> | <ul> | ||
| − | <li>Include an image of the PSF sample | + | <li>Include an image of the PSF sample indicating which beads were used for resolution measurement..</li> |
</ul> | </ul> | ||
<li>Analysis and Results</li> | <li>Analysis and Results</li> | ||
| Line 33: | Line 33: | ||
<ul> | <ul> | ||
<li>Show an example frame from the stability movie.</li> | <li>Show an example frame from the stability movie.</li> | ||
| − | <li> | + | <li>Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.) |
| − | + | ||
</ul> | </ul> | ||
<li>Analysis and Results</li> | <li>Analysis and Results</li> | ||
<ul> | <ul> | ||
| + | <li>Plot MSD versus time interval τ for individual and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.</li> | ||
<li>Provide a bullet point outline of data analysis methodology.</li> | <li>Provide a bullet point outline of data analysis methodology.</li> | ||
<li>Include a thorough discussion of error sources.</li> | <li>Include a thorough discussion of error sources.</li> | ||
Revision as of 21:58, 20 October 2015
- Resolution
- Procedure
- Document the samples you used and how you captured images (camera settings, software used, etc…)
- Data
- Include an image of the PSF sample indicating which beads were used for resolution measurement..
- Analysis and Results
- Report the resolution you measured. Make sure to include N and a measure of uncertainty.
- Show sample Gaussian fits.
- Explain the Matlab algorithm used for data analysis.
- Discussion
- Compare the measured value to the theoretical value.
- Include a thorough discussion of error sources. Do not comment on insignificant sources of error. To determine which error sources are significant, and which are not, you must think carefully about the uncertainty related to each error source and estimate its magnitude and sign. Include these estimates in your report along with your estimate of the combined, total uncertainty.
- Procedure
- Stability
- Procedure
- Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)
- Data
- Show an example frame from the stability movie.
- Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)
- Analysis and Results
- Plot MSD versus time interval τ for individual and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.
- Provide a bullet point outline of data analysis methodology.
- Include a thorough discussion of error sources.
- Discussion
- What are the benefits and drawbacks of differential tracking?
- Procedure
