Difference between revisions of "Optical Microscopy: Part 2 Report Outline"

Revision as of 19:23, 3 February 2015

• Update the apparatus section of your report to reflect the changes you made in part 2.

4. Fluorescence imaging and flat-field correction
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
   2. Data
   • Include raw images of two sizes of beads, the stained cell sample, reference image(s), and dark image(s).
   • It may be clearer to show the reference image as a surface plot.
   3. Analysis and Results
   • Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the improfile command in MATLAB.)
   • Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.
   • For the bead samples on the one hand, and for the cell samples on the other hand, include histograms of the sample, reference, dark, and corrected images on a single plot of log(count) vs intensity. (You may plot the mere envelop of the histogram, for clarity purposes.)
   • Document the steps you used to process the image.
   4. Discussion
   • How did your beam expander design affect your images?
5. Disrupting and imaging the actin network
   1. Procedure
      • Document sample preparation, including difficulties, and recording conditions (particularly exposure time and gain selected in the camera settings)
   2. Data
   • Include images of mouse embryonic fibroblasts + and - CytoD.
   3. Analysis and Results
   • What contrast do you unveil between the + and - CytoD conditions?
   4. Discussion
   • Can you quantify the morphological and physiological effect of CytoD treatment? What parameters would you choose to measure and report?