Difference between revisions of "Optical Microscopy: Part 2 Report Outline"
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<li>Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the <tt>improfile</tt> command in MATLAB.)</li> | <li>Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the <tt>improfile</tt> command in MATLAB.)</li> | ||
<li>Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.</li> | <li>Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.</li> | ||
| − | <li>For the bead samples on the one hand, and for the cell | + | <li>For the bead samples on the one hand, and for the cell samples on the other hand, include histograms of the sample, reference, dark, and corrected images on a single plot of log(count) vs intensity. (You may plot the mere envelop of the histogram, for clarity purposes.) </li> |
<li>Document the steps you used to process the image.</li> | <li>Document the steps you used to process the image.</li> | ||
</ul> | </ul> | ||
Revision as of 19:19, 3 February 2015
- Update the apparatus section of your report to reflect the changes you made in part 2.
- Fluorescence imaging and flat-field correction
- Procedure
- Document the samples you used and how you captured images (camera settings, software used, etc…)
- Data
- Include raw images of two sizes of beads, the stained cell sample, reference image(s), and dark image(s).
- It may be clearer to show the reference image as a surface plot.
- Analysis and Results
- Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the improfile command in MATLAB.)
- Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.
- For the bead samples on the one hand, and for the cell samples on the other hand, include histograms of the sample, reference, dark, and corrected images on a single plot of log(count) vs intensity. (You may plot the mere envelop of the histogram, for clarity purposes.)
- Document the steps you used to process the image.
- Discussion
- How did your beam expander design affect your images?
- Procedure
