Difference between revisions of "Limits of Detection:Report Requirements"
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| − | + | * Before you go to the lab, read the [http://measure.mit.edu/~20.309/wiki/images/2/27/Optical_Trapping_Lab_Manual.pdf optical trapping lab manual] and the the [http://measure.mit.edu/~20.309/wiki/index.php?title=Lab_Manual:Atomic_Force_Microscopy_(AFM) Atomic Force Microscopy lab manual] | |
| − | + | *Attend an optical trap lab session during the week of 11/28-12/3. | |
| − | + | *Attend an AFM lab session during the week of 12/5-12/9. | |
| − | + | *The mini-lab report will be due at midnight on 12/14. | |
| − | + | *The report should be answer-book style, like a problem set. | |
| − | + | *Your report should be in PDF format, submitted to Stellar prior to the deadline. Submit ALL of your computer code (.m files) you use in your analysis as a single zip file to Stellar. | |
| − | + | '''''You will gather data as a group; however, the report you submit must be entirely your own work. Everything in your report, including computer code, must be entirely your own work.''''' | |
| − | + | #Optical trap calibration | |
| − | + | ##What is your estimate of the trap stiffness as a function of power as determined by the three different calibration methods (equipartition, PSD roll-off, and Stokes drag)? | |
| − | + | ##Which method is superior? Explain why. | |
| − | # | + | ##What is the minimum detectable force as a function of power and bandwidth? |
| − | # | + | #AFM |
| − | # | + | ##What is the stiffness of the cantilever you measured? |
| − | + | ##How does the stiffness compare to the value computed from the cantilever's material properties? | |
| − | + | ##What is the smallest detectable force using that cantiliver? | |
| − | # | + | ##How you could modify the cantilever design to detect smaller forces? What is the smallest detectable force that could practically be detected using a silicon nitride cantilever? |
| − | # | + | ##How does the minimum detectable force compare to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)? |
| − | # | + | |
| − | + | ||
| − | + | ||
Revision as of 03:20, 1 December 2011
- Before you go to the lab, read the optical trapping lab manual and the the Atomic Force Microscopy lab manual
- Attend an optical trap lab session during the week of 11/28-12/3.
- Attend an AFM lab session during the week of 12/5-12/9.
- The mini-lab report will be due at midnight on 12/14.
- The report should be answer-book style, like a problem set.
- Your report should be in PDF format, submitted to Stellar prior to the deadline. Submit ALL of your computer code (.m files) you use in your analysis as a single zip file to Stellar.
You will gather data as a group; however, the report you submit must be entirely your own work. Everything in your report, including computer code, must be entirely your own work.
- Optical trap calibration
- What is your estimate of the trap stiffness as a function of power as determined by the three different calibration methods (equipartition, PSD roll-off, and Stokes drag)?
- Which method is superior? Explain why.
- What is the minimum detectable force as a function of power and bandwidth?
- AFM
- What is the stiffness of the cantilever you measured?
- How does the stiffness compare to the value computed from the cantilever's material properties?
- What is the smallest detectable force using that cantiliver?
- How you could modify the cantilever design to detect smaller forces? What is the smallest detectable force that could practically be detected using a silicon nitride cantilever?
- How does the minimum detectable force compare to typical forces in biological systems (e.g. antibody/antigen binding, DNA hybridization, interdomain forces in proteins, etc.)?
