DNA Melting Report Requirements for Part 1

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20.309: Biological Instrumentation and Measurement

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Report requirements

  1. Report submittal format:
    Size of your lab report file submitted MUST be less than 20 MB.
    Post only 1 single .pdf document, with code included in the pdf at the end, not separately.
    Include last name of each group member in the filename of the pdf you post. We would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.
  2. Report your signal to noise measurement and comment.
  3. Plot your favorite melting curves.
  4. For at least one of your data curves (or an average of your best curves), use your code from the simulation to perform a least-squares fit and comment on the results.
    • You may want to review the DNA Melting: Simulating DNA Melting - Basics wiki page.
    • What values do you obtain for ΔH and ΔS? Do you trust these values?
    • How would you adjust the model to make a better fit to your data?
    • Briefly discuss the issues with the data, speculate on underlying causes and suggest corrective actions.
    • Estimate the melting temperature based on the plot.

Lab manual sections