DNA Melting Report Requirements for Part 1

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20.309: Biological Instrumentation and Measurement

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Report requirements

  1. Report submittal format
    Size of your lab report file submitted MUST be less than 20 MB
    Post only pdf, with code included in the pdf at the end, not separately
    Include last name of each group member in the filename of the pdf you post. I would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.
  2. Report your signal to noise measurement and comment.
  3. Plot your favorite melting curves.
  4. For at least one of those curves (or an average of your best curves), use your code from the simulation to perform a least squares fit and comment on the results.
    1. What values do you obtain for ΔH and ΔS? Do you trust these values? How would you adjust the model to make a better fit to your data?

Lab manual sections