DNA Melting Report Requirements for Part 1

Report requirements

One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example: CrickFranklinWatson.pdf.
The file must be less than 20 MB.
Include code at the end of the document in an appendix, in the same pdf file, not as as separate upload.

Document your circuit design, optical design, and any ways that your instrument differs from the system described in the lab manual.
- For example, record your choice of resistors, capacitors and lenses, as well as a diagram of your chosen optical components.
- If you have not modified the circuits from their form in the report, you do not need to include the schematic in your report.
- Include a picture of your instrument.
Report your signal to noise measurement.
Plot at least one melting curve.
- The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
- On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
- Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters. You may use nlinfit to choose the best-fit parameters or you may choose them manually.
- Include a legend.
Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab.
- State the acceptance/rejection criteria for any hypotheses tests you will use.

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