DNA Melting Report Requirements for Part 1

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Report requirements

  • One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example: CrickFranklinWatson.pdf.
  • The file must be less than 20 MB.
  • Include code at the end of the document in an appendix.

Part 1 report outline

  1. Document your circuit design, optical design, and any changes you made to the system described in the lab manual.
  2. Plot at least one melting curve.
    • The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
    • On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
    • Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters.
    • Include a legend.
  3. Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
  4. Report your signal to noise measurement.

Lab manual sections

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