Difference between revisions of "DNA Melting Report Requirements for Part 1"

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(Report requirements)
(Report requirements)
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==Report requirements==
 
==Report requirements==
 
# Report submittal format
 
# Report submittal format
#;Size of your lab manual file submitted MUST be less than 20 MB
+
#;Size of your lab report file submitted MUST be less than 20 MB
 
#;Post only pdf, with code included in the pdf at the end, not separately
 
#;Post only pdf, with code included in the pdf at the end, not separately
 
#;Include last name of each group member in the '''filename''' of the pdf you post. I would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.
 
#;Include last name of each group member in the '''filename''' of the pdf you post. I would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.

Revision as of 14:51, 10 October 2012

20.309: Biological Instrumentation and Measurement

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Report requirements

  1. Report submittal format
    Size of your lab report file submitted MUST be less than 20 MB
    Post only pdf, with code included in the pdf at the end, not separately
    Include last name of each group member in the filename of the pdf you post. I would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.
  2. Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance.
  3. Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out.
    1. What is the signal to noise ratio (SNR) in units of dB for your system?
    2. Ensure that your system has an SNR of at least 12 dB.
  4. Obtain a DNA melting curve by plotting fluorescence versus temperature.
    1. Repeat two more times.
    2. On one graph, plot your three melting curves.
  5. Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit and comment on the results.
    1. What values do you obtain for ΔH and ΔS? Do you trust these values?

Lab manual sections