Difference between revisions of "DNA Melting Report Requirements for Part 1"
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| − | + | Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. Using the | |
| + | same amplifier gain, estimate the change in signal between DNA sample in and sample out. What is the signal | ||
| + | to noise ratio (SNR) in units of dB for your system? | ||
| + | Ensure that your system has an SNR of at least 12 dB. | ||
| + | Obtain a DNA melting curve by plotting fluorescence versus temperature. Repeat two more times. On the | ||
| + | same graph, plot your three melting curves. | ||
| + | Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares | ||
| + | fit. What values do you obtain for ΔH and ΔS? | ||
==Lab manual sections== | ==Lab manual sections== | ||
Revision as of 23:38, 2 December 2011
Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. Using the
same amplifier gain, estimate the change in signal between DNA sample in and sample out. What is the signal
to noise ratio (SNR) in units of dB for your system?
Ensure that your system has an SNR of at least 12 dB.
Obtain a DNA melting curve by plotting fluorescence versus temperature. Repeat two more times. On the
same graph, plot your three melting curves.
Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares
fit. What values do you obtain for ΔH and ΔS?
Lab manual sections
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
