Difference between revisions of "DNA Melting Report Requirements for Part 1"

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(Part 1 report outline)
(Part 1 report outline)
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# Report your signal to noise measurement.
 
# Report your signal to noise measurement.
 
# Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab.
 
# Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab.
 +
#* State the acceptance/rejection criteria for any hypotheses tests you will use.
  
 
==Lab manual sections==
 
==Lab manual sections==

Revision as of 13:43, 10 May 2015


Report requirements

  • One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example: CrickFranklinWatson.pdf.
  • The file must be less than 20 MB.
  • Include code at the end of the document in an appendix.

Part 1 report outline

  1. Document your circuit design, optical design, and any ways that your instrument differs from the system described in the lab manual.
  2. Plot at least one melting curve.
    • The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
    • On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
    • Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters.
    • Include a legend.
  3. Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
  4. Report your signal to noise measurement.
  5. Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab.
    • State the acceptance/rejection criteria for any hypotheses tests you will use.

Lab manual sections

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