Difference between revisions of "Assignment 8 Overview: flow channel & two-color microscope"
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With this goal in mind, the focus of this week's assignment is to implement a few upgrades to our microscopes, and incorporate the fluidic device and control components. It is broken into three parts: | With this goal in mind, the focus of this week's assignment is to implement a few upgrades to our microscopes, and incorporate the fluidic device and control components. It is broken into three parts: | ||
| − | # Part 1: fabricate a microfluidic device; | + | # [[Assignment 8, Part 1: fabricate a microfluidic device| Part 1: fabricate a microfluidic device]]; |
| − | # Part 2: implement 2-color imaging; | + | # [[Assignment 8, Part 2: build a two-color microscope| Part 2: implement 2-color imaging]]; |
| − | # Part 3: test your device with fluorescein. | + | # [[Assignment 8, Part 3: add flow control and test your device| Part 3: test your device with fluorescein]]. |
| − | Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment8.pdf. | + | WARNING: this assignment is heavy on the in-lab component. Make sure to start early to make sure you have enough time to get everything built! |
| + | |||
| + | Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment8.pdf. | ||
==References== | ==References== | ||
Revision as of 18:42, 30 October 2018
Overview
In Assignments 6 and 7, you familiarized yourselves with the basic tools and measurement techniques used in electronics. In the remaining assignments this semester, we'll work towards measuring the osmotic shock response of yeast cells. We'll build a few circuits, but more importantly, we'll apply the framework that we've learned - using transfer functions, feedback, and fourier transforms - to understand a biological system.
Our experiments are based off of research published in Science by MIT researchers in 2008 [1]. The basic idea is that we will use a microfluidic device to flow either regular or high-salt media over yeast cells, and measure the nuclear localization of the protein Hog1 using our fluorescence microscopes. We'll oscillate the flow of media from low to high salt at varying frequencies, and measure the amplitude and phase of Hog1's response.
With this goal in mind, the focus of this week's assignment is to implement a few upgrades to our microscopes, and incorporate the fluidic device and control components. It is broken into three parts:
- Part 1: fabricate a microfluidic device;
- Part 2: implement 2-color imaging;
- Part 3: test your device with fluorescein.
WARNING: this assignment is heavy on the in-lab component. Make sure to start early to make sure you have enough time to get everything built!
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment8.pdf.
References
- ↑ J. T. Mettetal, D. Muzzey, C. Gomez-Uribe, and A. van Oudenaarden, “The Frequency Dependence of Osmo-Adaptation in Saccharomyces cerevisiae,” Science, vol. 319, no. 5862, pp. 482–484, 2008
- Overview
- Part 1: feedback systems
- Part 2: fabricate a microfluidic device
- Part 3: add flow control and test your device
