Difference between revisions of "Assignment 2: fluorescence microscopy"
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{{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | {{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | ||
− | Part 1 | + | Part 1 ('''individually''') |
# What percentage of the results fall with one, two, and three standard deviations? Is it reasonable to approximate this situation as a normal distribution? | # What percentage of the results fall with one, two, and three standard deviations? Is it reasonable to approximate this situation as a normal distribution? | ||
# Produce a log-log plot of standard deviation of the number of photons emitted as a function of the average number of photons emitted for probability of photon emission equal to the values: 1E-6, 1E-5, 1E-4, 1E-3, and 1E-2. '''Hints:''' | # Produce a log-log plot of standard deviation of the number of photons emitted as a function of the average number of photons emitted for probability of photon emission equal to the values: 1E-6, 1E-5, 1E-4, 1E-3, and 1E-2. '''Hints:''' | ||
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# In what situations is dark current noise a significant problem? | # In what situations is dark current noise a significant problem? | ||
− | Parts 2 & 3 | + | Parts 2 & 3 ('''as a team''') |
# Choose focal lengths for L3, L4 and L5 so that the laser beam diameter matches the FOV of the CCD camera. | # Choose focal lengths for L3, L4 and L5 so that the laser beam diameter matches the FOV of the CCD camera. | ||
# Sketch a block diagram of the illumination path. Make sure to indicate | # Sketch a block diagram of the illumination path. Make sure to indicate |
Revision as of 00:09, 25 January 2018
This assignment has 3 parts.
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment2.pdf.
- Overview
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
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